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We describe techniques for identifying functional promoter elements in the 5'-flanking region of the murine cyclic nucleotide phosphodiesterase 3B (mPDE3B) gene. The 5'-flanking region of the mPDE3B gene was cloned and sequenced, and putative transcription factor binding sites were identified with computational tools. A series of reporter plasmids containing the luciferase gene fused to different

OBJECTIVE: The serotonin system has long been of interest in biological models of human personality. The purpose of this positron emission tomography (PET) study was to search for relationships between serotonin 5-HT(1A) receptor density and personality traits. METHOD: Fifteen normal male subjects, ages 20-45 years, were examined with PET and the radioligand [(11)C]WAY100635. Personality traits we

Levels of mRNA for c-fos, nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), TrkB, and TrkC were studied using in situ hybridization in the rat brain at different reperfusion times after unilateral middle cerebral artery occlusion (MCAO). Short-term (15 min) MCAO, which does not cause neuronal death, induced elevated BDNF mRNA expression confined to ipsilat

BACKGROUND: Cathelicidins are a group of antibiotic peptides with broad antimicrobial activity. They are considered to be an essential part of the innate immune system. The only known human cathelicidin is the human cationic antimicrobial protein (hCAP-18), from which the antimicrobial peptide LL-37 is released. METHODS AND RESULTS: In the present study, we purified hCAP-18 from seminal plasma and

Lentiviral vectors containing recombinant envelope proteins that target hematopoietic stem cells have now been engineered to increase the efficiency of gene transfer.

OBJECTIVE: Coronary artery disease is a prevalent cause of morbidity and mortality in diabetes. Little is known about insulin-like growth factor-I receptors (IGF-IR) and insulin receptors (IR) in human coronary endothelium. Our aim was to characterize IGF-IR and IR in human coronary artery endothelial cells (HCAEC). DESIGN: Cultured human coronary artery endothelial cells were used. Gene expressio

The impact of the optical properties on the annual performance of flat plate collectors in a Swedish climate has been estimated with the MINSUN program. The collector parameters were determined with a theoretically based calculation program verified from laboratory measurements. The importance of changes in solar absorptance and thermal emittance of the absorber, the addition of a teflon film or a

Arbuscular mycorrhizal (AM) cucumber seedlings and uncolonized controls were grown in growth chambers which allowed separation of compartments with roots from compartments with the extraradical mycelium alone. Two fungi, Glomus invermaium Hall and G. caledonium (Nicol. and Gerd.) Trappe and Gerdemann, were used. Bacterial numbers (direct and viable count) and activities (thymidine incorporation) w

The results of a study on the phase behavior of the monoolein/water system with the addition of the hydrocarbon 1,8-cineole (C10H18O) and supercritical carbon dioxide at 15 MPa is presented. To two mixtures consisting of 9 and 18 wt.% water in monoolein, 5, 10, and 20 wt.% cineole were added and heated to between 35 and 80 degreesC at atmospheric pressure and in the presence of supercritical carbo

Endothelin-1 has been shown to contribute to basal vascular tone in man. Since endothelin-1 is a potent vasoconstrictor putatively involved in hypertension, we have compared the contractile responses of endothelin-1 and noradrenaline in relation to potassium chloride in subcutaneous resistance arteries from subjects with established essential hypertension with matched controls. Furthermore, with R

This paper presents a novel tool to be used for signature verification. The method is based on the vector context matrix concept that enables robust and precise extraction of the point correspondences between a sample and a database model. Using a digitizing tablet as a source for input, information of the pressure, speed and pen inclination is obtained in addition to the coordinates of the sample

TCR signaling is mediated by intracellular signaling molecules and nuclear transcription factors, which are tightly regulated by interaction with regulatory proteins such as Grb2 and SLAP. We reported recently that TCR stimulation induces the expression of cytokine-induced SH2 protein (CIS). The expression of CIS promotes TCR-mediated activation. We have now found specific interactions between CIS