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Modification of bovine heart succinate dehydrogenase with ethoxyformic anhydride and Rose bengal: Evidence for essential histidyl residues protectable by substrates

Purified and membrane-bound succinate dehydrogenase (SDH) from bovine heart mitochondria was inhibited by the histidine-modifying reagents ethoxyformic anhydride (EFA) and Rose Bengal in the presence of light. Succinate and competitive inhibitors protected against inhibition, and decreased the number of histidyl residues modified by EFA. The essential residue modified by EFA was not the essential

Virtual entrepreneurs : Boundary spanning between the private and the public spheres in the entrepreneuring process

This article is located within the context of women entrepreneurs involved in the process of virtual enterprising. It examines the process of structure building as an embedding process from a constructivist perspective. The research aims to ask whether entrepreneurship is a boundless process embedded in family and firm at the same time and how they capitalized on structuring resources embedded in

Towards Active Learning Spaces and the Flipped Classroom Model

It has been acknowledged that the traditional didactic lecture does not always provide the ideal learning and teaching experience (Bligh, 1998). Over the last ten years, individuals and institutions have been exploring the pedagogical possibilities of providing more active and engaging alternatives. One model in particular has been influential in sparking change. The use of Active Learning Spaces

Testing for Occupational Crowding in Eighteenth-Century British Agriculture

In the unskilled labor market of Industrial Revolution Britain, there was a distinct division of labor between the sexes. This occupational sorting may have been caused by gender discrimination, but, because men and women had different comparative advantages, it could also have been produced by a competitive market. This paper attempts to determine whether the division of labor resulted from discr

The structural gene for aspartokinase II in Bacillus subtilis is closely linked to the sdh operon

The aecA and aecB loci map at 250 and 290 degrees, respectively, on the Bacillus subtilis chromosomal genetic map. The aecB locus has been proposed as the structural gene for aspartokinase II. From DNA sequence analyses and comparisons to the sequence of the aspartokinase II gene, it can be concluded that the structural gene for aspartokinase II is located close to sdh at 250 degrees and cannot be

New properties of Bacillus subtilis succinate dehydrogenase altered at the active site

Mammalian and Escherichia coli succinate dehydrogenase (SDH) and E. coli fumarate reductase apparentlycontain an essential cysteine residue at the active site, as shown by substrate-protectable inactivation withthiol-specific reagents. Bacillus subtilis SDH was found to be resistant to this type of reagent and containsan alanine residue at the amino acid position equivalent to the only invariant c

Radiocarbon calibration uncertainties during the last deglaciation : Insights from new floating tree-ring chronologies

Radiocarbon dating is the most commonly used chronological tool in archaeological and environmental sciences dealing with the past 50,000 years, making the radiocarbon calibration curve one of the most important records in paleosciences. For the past 12,560 years, the radiocarbon calibration curve is constrained by high quality tree-ring data. Prior to this, however, its uncertainties increase rap

Astrocytic expression of GSTA4 is associated to dopaminergic neuroprotection in a rat 6-OHDA model of Parkinson’s disease

Idiopathic Parkinson’s disease (PD) is a complex disease caused by multiple, mainly unknown, genetic and environmental factors. The Ventral root avulsion 1 (Vra1) locus on rat chromosome 8 includes the Glutathione S-transferase alpha 4 (Gsta4) gene and has been identified in crosses between Dark Agouti (DA) and Piebald Virol Glaxo (PVG) rat strains as being associated to neurodegeneration after ne

Observing femtosecond fragmentation using ultrafast X-ray-induced auger spectra

Molecules often fragment after photoionization in the gas phase. Usually, this process can only be investigated spectroscopically as long as there exists electron correlation between the photofragments. Important parameters, like their kinetic energy after separation, cannot be investigated. We are reporting on a femtosecond time-resolved Auger electron spectroscopy study concerning the photofragm

rt-muse : measuring real-time characteristics of execution platforms

Operating systems code is often developed according to principles like simplicity, low overhead, and low memory footprint. Schedulers are no exceptions. A scheduler is usually developed with flexibility in mind, and this restricts the ability to provide real-time guarantees. Moreover, even when schedulers can provide real-time guarantees, it is unlikely that these guarantees are properly quantifie

Colloidal assembly and 3D shaping by dielectrophoretic confinement

For decades, scientists and engineers have strived to design means of assembling colloids into ordered structures. By now, the literature is quite peppered with reports of colloidal assemblies. However, the available methods can assemble only a narrow range of structures or are applicable to specific types of colloids. There are still only few generic methods that would lead to arbitrary colloidal

Modulation of electrostatic interactions to reveal a reaction network unifying the aggregation behaviour of the Aβ42 peptide and its variants

The aggregation of the amyloid β peptide (Aβ42), which is linked to Alzheimer's disease, can be altered significantly by modulations of the peptide's intermolecular electrostatic interactions. Variations in sequence and solution conditions have been found to lead to highly variable aggregation behaviour. Here we modulate systematically the electrostatic interactions governing the aggregation kinet

Measurement of the higher-order anisotropic flow coefficients for identified hadrons in Au + Au collisions at sNN =200 GeV

Measurements of the anisotropic flow coefficients v2{Ψ2},v3{Ψ3},v4{Ψ4}, and v4{Ψ2} for identified particles (π±,K±, and p+p) at midrapidity, obtained relative to the event planes Ψm at forward rapidities in Au + Au collisions at sNN=200GeV, are presented as a function of collision centrality and particle transverse momenta pT. The vn coefficients show characteristic patterns consistent with hydrod

Early repolarization in children with unexplained syncope

Introduction: It has traditionally been believed that early repolarization (ER) is benign. Significant association between ER and sudden cardiac arrest due to idiopathic ventricular fibrillation was recently found in a large cohort of adult survivors of sudden cardiac arrest. In some prior studies, unexplained syncope has been linked to risk of sudden death, but the mechanisms remain speculative.W