Isolation of Mitochondria
Although it is possible to measure respiration by intact tissues it is often useful to be able to isolate uncontaminated, intact and functional mitochondria. Here, we will consider some of the principles for isolating cell organelles. This discussion is not meant to be exhaustive nor will it be a cookbook with recipes to be followed step-by-step. Useful general references are Douce 1985, Møller et