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Isolation and characterization of two European strains of Ehrlichia phagocytophila of equine origin

We report the isolation and partial genetic characterization of two equine strains of granulocytic Ehrlichia of the genogroup Ehrlichia phagocytophila. Frozen whole-blood samples from two Swedish horses with laboratory-verified granulocytic ehrlichiosis were inoculated into HL-60 cell cultures. Granulocytic Ehrlichia was isolated and propagated from both horses. DNA extracts from the respective st

Video-assisted gastrostomy in infants less than 1 year.

The objectives of this study were to report our experience with the laparoscopic video-assisted gastrostomy technique in infants operated during their first year of life. A total of 53 infants (35 males, 18 females) aged 6 +/- 3 months, varying from 3 weeks to 11 months, underwent video-assisted gastrostomy. They were prospectively followed up. Included are infants with neurological dysfunction, c

Representativity of a postal public health questionnaire survey in Sweden, with special reference to ethnic differences in participation

AIM: Non-participation in health surveys is a common phenomenon. When differences between participants and non-participants are considerable, the external validity of the sample survey may decrease and false conclusions might be drawn about the health status of the population. For this reason, the authors aimed to investigate the representativity of a postal questionnaire survey performed in the c

Crystal structures of cementitious compounds - Part 3: Calcium sulfates

The crystal structures of the following calcium sulfate phases are reviewed: CaSO4.2H(2)O (C (S) over barH(0.5), monoclinic); CaSO4.0.6H(2)O (C (S) over barH(0.6), pseudotrigonal, monoclinic); CaSO4.0.5H(2)O (C (S) over barH(0.5), monoclinic); CaSO4 (111, CS, pseudohexagonal, orthorhombic), CaSO4 (II, CS, orthorhombic). Drawings of the atomic structures, crystal data and literature references are

Several interacting genes influence the malignant hyperthermia phenotype

Malignant hyperthermia (MH), a potentially lethal disorder of skeletal muscle calcium homeostasis, manifests only on exposure to certain anaesthetic drugs. The mode of inheritance appears to be autosomal dominant with both locus and allelic heterogeneity having been reported. Association analysis of eight MH candidate loci in UK families has indicated that several genes influence susceptibility in

Dynamics of protein and peptide hydration

Biological processes often involve the surfaces of proteins, where the structural and dynamic properties of the aqueous solvent are modified. Information about the dynamics of protein hydration can be obtained by measuring the magnetic relaxation dispersion (MRD) of the water 2 H and 17 0 nuclei or by recording the nuclear Overhauser effect (NOE) between water and protein protons. Here, we use the

Highly diastereoselective hydrogenations leading to ß-hydroxy ∂-lactones in hydroxy-protected form. A modified view of ∂-lactone conformations.

Enol MEM ethers 4 and 15 and the corresponding enol acetates were hydrogenated over Pd/C with very high (>99%) diastereoselectivity to saturated -lactones. A stereochemical generalization can be formulated thus: trans-5,6-disubstituted 1-oxa-3-cyclohexen-2-ones (e.g. 14 and 15) are hydrogenated over Pd with high selectivity from the side trans to the C(6)-substituent. A mechanistic rationalization

Palearctic Sympiesis acalle and Sympiesis gordius (Hymenoptera : Eulophidae) in North America: Taxonomic changes and a review of Nearctic host records

The Palearctic eulophids, Sympiesis acalle (Walker) and S. gordius (Walker), are widespread in North America where they have been known as S. bimaculatipennis Girault and S. marylandensis Girault. respectively. To update their taxonomy, we have established seven new synonyms: Astichus bimaculatipennis Girault, Sympiesis bimaculata Crawford, and S. meteori Girault = S. acalle (Walker); and, S. mary

NIKE: a new clinical tool for establishing levels of indications for cataract surgery

Purpose: The purpose of this study was to construct a new clinical tool for establishing levels of indications for cataract surgery, and to validate this tool. Methods: Teams from nine eye clinics reached an agreement about the need to develop a clinical tool for setting levels of indications for cataract surgery and about the items that should be included in the tool. The tool was to be called 'N

Influence of ignition sources on heat release rate in the furniture calorimeter

When measuring the production rates of heat and smoke of upholstered furniture the ignition source must not influence the test results. In this paper variations in the strength, application time and geometry of the ignition burner used in the furniture calorimeter are studied. Results from replicate furniture calorimeter chair test show that the burning behaviour is almost independent of the burne