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Quality Control for high-throughput Quantitative Proteomics - Harnessing the potential of label-free LC-MS

Popular Abstract in Swedish Sedan kartläggningen av det mänskliga genomet, har mer fokus lagts på dess produkt, proteomet, som definieras som de proteiner som uttrycks i en cell vid en viss tidpunkt. Proteomets dynamiska egenskaper gör det till en fantastisk källa för så kallade biomarkörer, det vill säga molekyler som genom deras koncentrationsmönster kan användas för att särskilja sjuka och frisMultiplex protein quantification, proteomics, is essential for uncovering new biomarkers and understanding biology. Liquid chromatography coupled to mass spectrometry (LC-MS) is the predominant technique for these measurements. In this thesis, optimization and quality control strategies for different phases of the LC-MS pipeline have been developed. Due to the inherent complexity and dynamic rang

Fragments of home in youth care institutions

A youth care institution may seem like a sort of regulated home. It does not resemble a home in the sense of a private household, and the youth living there usually do not define it as a home, but it seems like a home in the sense of being a place where youth in treatment spend most of their days and nights. But is that what a home is? In many cases, people in modern societies are not supposed to

A hydrogeological study of the Nhandugue River, Mozambique - A major groundwater recharge zone

The Nhandugue River flows over the western margin of the Urema Rift, the southernmost extension of the East African Rift System, and marks the north-western border of Gorongosa National Park, Mozambique. It constitutes one of the major indispensable water resources for the ecosystem that the park protects. Our study focused on the hydrogeological conditions at the western rift margin by resistivit

The effects of temperature on avian physiology, behavior and development

Popular Abstract in Swedish Omgivningens temperatur är kanske en av de faktorer som i allra störst utsträckning påverkar djur och deras livsmiljö. Detta bottnar givetvis till stor del i temperaturens effekt på naturen. Betänk till exempel årstidsväxlingarna; att vintern blir till vår, och att hösten (i samband med de första snöfallen) allt mer skrider mot ännu en kall och vit vinter bestäms och reTemperature is a property of profound matter in all aspects of animal life, affecting both individual energy budgets and the environments in which animals reside. Thus, a proper understanding of the effects of temperature variation is instrumental in understanding not only animal form and function, but also the structure and dynamics of wild populations. Accordingly, the aims of this thesis were t