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Bacterial ferrochelatase turns human: Tyr13 determines the apparent metal specificity of Bacillus subtilis ferrochelatase.

Ferrochelatase catalyzes the insertion of Fe(2+) into protoporphyrin IX. The enzymatic product heme (protoheme IX) is a well-known cofactor in a wide range of proteins. The insertion of metal ions other than Fe(2+) occurs rarely in vivo, but all ferrochelatases that have been studied can insert Zn(2+) at a good rate in vitro. Co(2+), but not Cu(2+), is known to be a good substrate of the mammalian

Study of the micelle-to-vesicle transition and smallest possible vesicle size by temperature-jumps.

We have investigated the temperature induced micelle-to-vesicle transition in a binary non-ionic surfactant/water system, for which the spontaneous curvature decreases with increasing temperature. Temperature jumps with variable rate were performed in a microwave oven, from a micellar phase at 5 ◦C to a lamellar phase region at 35 ◦C, passing a liquid-liquid two phase region where dilute and conce

Morphological and functional changes in multifocal vitelliform retinopathy and biallelic mutations in BEST1.

Purpose: To describe morphological and functional changes in a single patient with multifocal Best vitelliform macular dystrophy (BVMD) and to perform a genotype/phenotype correlation. Methods: The proband with multifocal BVMD and three of her family members were examined with electrooculography (EOG), full-field electroretinography (full-field ERG), multifocal electroretinography (mfERG) and opti

Mesopredator release by an emergent superpredator: A natural experiment of predation in a three level guild

Background: Intraguild predation (IGP) is widespread but it is often neglected that guilds commonly include many layers of dominance within. This could obscure the effects of IGP making unclear whether the intermediate or the bottom mesopredator will bear higher costs from the emergence of a new top predator. Methodology/Principal Findings: In one of the most extensive datasets of avian IGP, we an

The prognostic value of Ki67 is dependent on estrogen receptor status and histological grade in premenopausal patients with node-negative breast cancer.

The aim of this study was to evaluate the prognostic value of Ki67 in relation to established prognostic factors in lymph node-negative breast cancer, and furthermore, whether the prognostic impact was dependent on estrogen receptor (ER) status and histological grade. In 200 premenopausal patients, with 5 years of follow-up, Ki67 was determined on tissue microarrays. In univariate analysis, Ki67 (

Role of wave velocity for encountered wave statistics

Encountering waves overtaking a ship sailing on the ocean may cause instability. Therefore it is of great importance to study the properties of such waves, for example slope, wavelength and amplitude. When making statistics on the properties of overtaking waves, only the waves that are faster than the ship will affect the resulting distribution. Thus the velocity of the individual waves plays an i

A simple rate law experiment using a custom-built isothermal heat conduction calorimeter

Most processes (whether physical, chemical, or biological) produce or consume heat: measuring thermal power (the heat production rate) is therefore a typical method of studying processes. Here we describe the design of a simple isothermal heat conduction calorimeter built for use in teaching; we also provide an example of its use in simultaneously determining the rate constant and the reaction ent

Research in motion: the enigma of Parkinson's disease pathology spread.

Neuropathological changes in Parkinson's disease progress slowly and spread according to a characteristic pattern. Recent papers have shed light on this progression of pathology by examining the fate of neurons grafted into the brains of patients with Parkinson's disease. Two of these studies demonstrate that grafted healthy neurons can gradually develop the same pathology as host neurons in the d

Risk of sick leave and disability pension in working-age women and men with knee osteoarthritis.

OBJECTIVES: To investigate sick leave and disability pension in working-age subjects with knee osteoarthritis (OA) compared with the general population. METHODS: Population-based cohort study: individual-level inpatient and outpatient Skåne Health Care Register data were linked with data from the Swedish Social Insurance Agency. In 2009 all working-age (16-64 years) Skåne County residents who i