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Protein L from the bacterial species Peptostreptococcus magnus binds specifically to the variable domain of Ig light chains, without interfering with the antigen-binding site. In this work a genetically engineered fragment of protein L, including four of the repeated Ig-binding repeat units, was employed for the purification of Ig from various sources. Thus, IgG, IgM, and IgA were purified from hu

Protein L is an immunoglobulin light chain-binding protein expressed by some strains of the anaerobic bacterial species Peptostreptococcus magnus. The major variable region subgroups of human kappa and lambda light chains were tested for protein L binding; V kappa I, V kappa III, and V kappa IV bound protein L, whereas no binding occurred with proteins of the V kappa II subgroup or with any lambda

Protein G, an immunoglobulin (Ig)-binding protein isolated from group C or G streptococci, binds to the Fc portion of IgG. Protein L, from the anaerobic bacterium Peptostreptococcus magnus, specifically binds light chains of Ig. In this study, protein G and L were used to measure the production of antibodies in immunized rabbits. Two rabbits were immunized with a mixture of human urinary proteins

Protein G, an IgG-binding protein, purified from the surface of group G streptococci, was coupled to alkaline phosphatase. The conjugate was used for detection of polyclonal goat and rabbit antibodies and monoclonal mouse IgG1, IgG2a and IgG2b in an enzyme-linked immunosorbent assay. A two-step coupling procedure was used, in which glutaraldehyde was allowed to react with the enzyme, excess glutar

Using the newly described streptococcal surface protein, protein G, which has powerful immunoglobulin G binding properties, solid-phase radioimmunoassays were developed for the quantitation of goat and rat immunoglobulin G bound to the plastic surface of microtiter plates. The binding of goat immunoglobulin G to the surface via a specific antigen (guinea pig alpha 1-microglobulin) permitted the de

When museums of cultural heritage are no longer defined solely in terms of their collections and cultural environments, how much may they change before they cease to be museums and what do they become then? As a means of approaching this question this chapter focuses on two institutions of Swedish cultural heritage, The American Swedish Institute (ASI) in Minneapolis, Minnesota and Kulturen in Lun

I kapitlet är syftet att vidga perspektivet på regleringar och diskutera den omreglering som har skett i arbetslivet under de tre-fyra senaste decennierna. Förändringarna i arbetslivet hänger samman med nya konkurrensvillkor, ny teknik och förändrade arbetsorganisationer. Det handlar om en utveckling bort från det som brukar kallas industrisamhället till det post-industriella samhället. Hur påverk

Phylogenetic relationships within the helcionelloid molluscs have been difficult to establish. One of the reasons for this is that qualitative approaches to investigating morphological variation in this group have struggled to identify clear patterns. An alternative method of identifying these patterns is to study these organisms quantitatively. Here this approach is exemplified by employing morph

Breast tomosynthesis (BT) is an X-ray imaging technique recently introduced as an alternative or complement to standard digital mammography (DM) in breast imaging and breast cancer screening. In BT, a set of projection images is acquired over a limited angular range and reconstructed into a volume of slice images. The method includes many possible combinations of acquisition parameters that have t

Nomenclatural type definitions are one of the most important concepts in biological nomenclature. Being physical objects that can be re-studied by other researchers, types permanently link taxonomy (an artificial agreement to classify biological diversity) with nomenclature (an artificial agreement to name biological diversity). Two proposals to amend the International Code of Nomenclature for alg

The mechanisms responsible for macrovascular complications in diabetes remain to be fully understood. Recent studies have identified impaired vascular repair as a possible cause of plaque vulnerability in diabetes. This notion is supported by observations of a reduced content of fibrous proteins and smooth muscle cell mitogens in carotid endarterectomy from diabetic patients along with findings of

The purpose of this paper is to introduce the concept coworkers’ communicative responsibility taking and to test which factors might influence coworkers’ tendency to take communicative responsibility. The study is split into two phases where on exploratory phase consisted of an exploratory factor analysis on 2244 with the aim to find specific latent factors to test in the confirmatory phase. The c

NAD står för Nätverk, Aktivitet och Delaktighet och är ett samarbete mellan Länsstyrelsen, Region Skåne, Arbetsförmedlingen och NÄTVERKET Idéburen sektor Skåne, en regional intresseorganisation för civilsamhället i Skåne. NAD utvecklar och förmedlar aktiviteter som erbjuds som en del av den etableringsplan som Arbetsförmedlingen upprättar tillsammans med den nyanlända. NAD syftar till att underlät

Knowledge hubs are generally located in large and dynamic population clusters, but there is little empirical evidence on what has driven the location of services in the economy, particularly the knowledge-intensive ones that form these hubs. This column describes how the geography of services across the US has been influenced by the interaction between county and industry characteristics. The pres

Background: Tyrosine kinase inhibitors (TKIs) have improved the survival of patients with chronic myeloid leukaemia. Many patients have deep molecular responses, a prerequisite for TKI therapy discontinuation. We aimed to define precise conditions for stopping treatment. Methods: In this prospective, non-randomised trial, we enrolled patients with chronic myeloid leukaemia at 61 European centres i

A mixed-domain device characterisation algorithm with is proposed and evaluated. Wideband signals from a time-domain wavelet generator are applied to millimetre-wave antennas. Deliberately chosen gating techniques in time- A nd frequency-domain are used to relax the calibration effort to one reference measurement. A frequency-domain gate shapes the excitation pulse. Selective removal of setup arte