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Genetic Characterization of Bacillus subtilis odhA and odhB, encoding 2-oxoglutarate dehydrogenase and dihydrolipoamide transsuccinylase, respectively

The 2-oxoglutarate dehydrogenase complex consists of three different subenzymes, the E1o (2-oxoglutarate dehydrogenase) component, the E2o (dihydrolipoyl transsuccinylase) component, and the E3 (dihydrolipoamide dehydrogenase) component. In Bacillus subtilis, the E1o and E2o subenzymes are encoded by odhA and odhB, respectively. A plasmid with a 6.8-kilobase-pair DNA fragment containing odhA and o

Electron-paramagnetic-resonance spectroscopy of Bacillus subtilis cytochrome b558 in Escherichia coli membranes and in succinate dehydrogenase complex from Bacillus subtilis membranes

Cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex was studied by electron-paramagnetic-resonance (EPR) spectroscopy. The cytochrome amplified in Escherichia coli membranes by expression of the cloned cytochrome gene and in the succinate dehydrogenase complex immunoprecipitated from solubilized B. subtilis membranes, respectively, is shown to be low spin with a highly anisotr

Mindfulness based flourishing program: A cross-cultural study of Hong Kong Chinese and British participants

The Mindfulness Based Flourishing Program (MBFP) is an online 8-week intervention developed for enhancing wellbeing with the use of mindfulness practices, through targeting a range of positive variables. The efficacy of the MBFP has been demonstrated in a randomized controlled trial, and in order to further establish it as an intervention with widespread application, cross-cultural validation is w

Genetic and biochemical characterization of Bacillus subtilis mutants defective in expression and function of cytochrome b-558

Bacillus subtilis succinate dehydrogenase is bound to the cytoplasmic membrane by cytochrome b-558, a 23-kDa transmembrane protein which also functions as electron acceptor to the dehydrogenase. The structural gene for the apocytochrome, sdhC, has previously been cloned and sequenced. In this work the structure and translation of cytochrome b-558 was studied in different sdhC mutants. Mutant cytoc

Lactose, glucose and galactose content in milk, fermented milk and lactose-free milk products

Lactose, glucose and galactose contents in milk and fermented milk products and their lactose-free alternatives, were determined. Storage of products up to the best-before dates had no effect on carbohydrate composition. Total galactose content in yoghurt, i.e., free galactose plus the galactose moiety of lactose, was 2.3–2.4 g 100 g−1 (94–95% of that in milk), whereas that in Swedish soured milk

Roadmap and roadblocks for the band gap tunability of metal halide perovskites

Solar cells based on metal-halide perovskite semiconductors inspire high hopes for efficient low cost solar cell technology. This material class exhibits a facile tunability of the band gap making them interesting for multi-junction device technology. We here compare and highlight trends in the band gap tunability and device performance metrics in reported metal halide perovskite alloys of a wide

Syntheses and catalytic oxotransfer actiVIties of oxo molybdenum(VI) complexes of a new aminoalcohol phenolate ligand

The new aminoalcohol phenol 2,4-di-tert-butyl-6-(((2-hydroxy-2-phenylethyl)amino)methyl)phenol (H2L) was prepared by a facile solvent-free synthesis and used as a tridentate ligand for new cis-dioxomolybdenum(vi)(L) complexes. In the presence of a coordinating solvent (DMSO, MeOH, pyridine), the complexes crystallise as monomeric solvent adducts while in the absence of such molecules, a trimer wit

Measurement of the top quark mass in the tt̄ → Dilepton channel from √s=8 TeV ATLAS data

The top quark mass is measured in the tt̄ → dileptonchannel (lepton=e, µ) using ATLAS data recorded in the year 2012 at the LHC. The data were taken at a proton-proton centre-of-mass energy of √s=8 TeV and correspond to an integrated luminosity of about 20.2fb-1. Exploiting the template method, and using the distribution of invariant masses of lepton-b-jetpairs, the top quark mass is measured to b