The disaccharide 6-O-β-d-galactopyranosyl-2-acetamido-2-deoxy-d-galactose has been synthesized by transfer of the β-d-galactopyranosyl residue from lactose to 2-acetamido-2-deoxy-d-galactose utilizing the transferase activity of β-galactosidase from . To make the enzyme reusable, it was applied in an immobilized form covalently bound to Sepharose CL-4B. The yield of the disaccharide was about 20%,

Background: Biallelic variants in the major facilitator superfamily domain containing 8 gene (MFSD8) are associated with distinct clinical presentations that range from typical late-infantile neuronal ceroid lipofuscinosis type 7 (CLN7 disease) to isolated adult-onset retinal dystrophy. Classic late-infantile CLN7 disease is a severe, rare neurological disorder with an age of onset typically betwe

This study contributes to geothermal exploration in 1660–1520 Ma old, reworked bedrock in Sweden. Our primary objectives are to constrain the orientation of horizontal stresses, and to discuss implications for geothermal exploration. High-resolution acoustic televiewer image data reveal the downhole distribution of stress indicators (borehole breakouts, drilling-induced fractures and petal centrel

Multimetallic nanoparticles possess a variety of beneficial properties with potential relevance for various applications. These metallic nanoparticles can consist of randomly ordered alloys, which retain the properties of the constituting elements, or ordered intermetallics, which possess extended properties. Depending on the desired application, specific alloys or intermetallic compounds are requ

Microbial, algal, plant and animal cells have been immobilized, with preserved viability, by entrapment in various matrices according to a new bead polymerization technique. The cell polymer/monomer mixture is kept suspended in a hydrophobic phase such as soy, paraffin, or silicon oil, tri-n-butylphosphate, or dibutyphtalate, which is compatible with the cells. The various monomers or polymers tes

Topological superconductors are appealing building blocks for robust and reliable quantum information processing. Most platforms for engineering topological superconductivity rely on a combination of superconductors, materials with intrinsic strong spin-orbit coupling, and external magnetic fields, detrimental for superconductivity. We propose a setup where a conventional Josephson junction is lin

Glycosaminoglycans (GAGs) such as heparin and heparan sulfate (HS) play crucial roles in inflammation and wound healing, serving as regulators of growth factors and pro-inflammatory mediators. In this study, we investigated the influence of heparin/HS on thrombin proteolysis and its interaction with the generated 11 kDa thrombin-derived C-terminal peptides (TCPs). Employing various biochemical and

Aims. We aim to determine the influence of the Milky Way's warp on the kinematics of stars across the disc, and therefore measure its precession rate and line of nodes under different assumptions. Methods. We applied Jeans'first equation to a model of a rigidly precessing warp. The predictions of these models were fitted to the average vertical velocities of stars with measured line-of-sight veloc

We introduce a model of hadronization based on invertible neural networks that faithfully reproduces a simplified version of the Lund string model for meson hadronization. Additionally, we introduce a new training method for normalizing flows, termed MAGIC, that improves the agreement between simulated and experimental distributions of high-level (macroscopic) observables by adjusting single-emiss

We study a simple extension of the Standard Model featuring a dark sector with an ultralight pseudo-Nambu-Goldstone boson as a dark matter candidate. We focus on the mass range O(10-20-10-10) eV, relevant for strong gravity applications, and explore its production and evolution in the early Universe. The model is formulated in such a way that dark matter does not couple directly to photons or othe

Heterotopic ossification (HO) in tendons can lead to increased pain and poor tendon function. Although it is believed to share some characteristics with bone, the structural and elemental compositions of HO deposits have not been fully elucidated. This study utilizes a multimodal and multiscale approach for structural and elemental characterization of HO deposits in healing rat Achilles tendons at

Horse liver alcohol dehydrogenase was immobilized on glycerylpropyl-silica (10 μm, 1000-Å pores) activated with 2,2,2-trifluoroethanesulfonyl chloride (tresyl chloride). The coupling and activity yield was almost 100%. The coenzyme-binding sites were equivalent and virtually unaffected by the immobilization process, as judged from Scatchard plots and active-site titrations. The silica-bound enzyme

Lactate dehydrogenase has been purified by precipitation with a bis-ligand. The precipitating agent in this case was Bis-NAD. This approach of affinity precipitation is also applicable to other enzymes.

Magnetic calcium alginate yeast beads, made by incorporation of magnetite or colloidal magnetic liquid, FerrofluidTM, exhibited catalytic behavior similar to that of their nonmagnetic counterparts. The magnetic immobilized preparations’ shortterm performance, long-term operational stability, and capacity forin situ activation were unaffected by the inclusion of magnetic material. The magnetic qual

Concanavalin A was covalently bound to porous silica (100-1000 Å) by three different coupling procedures. The performance of the preparations was evaluated from their ability to resolve a mixture of closely related sugars. The Concanavalin A-silica was used for efficient and rapid (5-20 min) analysis/purification of samples containing the glucoproteins peroxidase and glucose oxidase. Such columns

Three methods were developed for preparing alginate gels containing cells that are stable in phosphate containing media. In Method I preformed alginate beads containing entrapped cells were treated with polyethyl eneimine followed by glutaraldehyde. In Method II alginate sol was treated with a carbodiimide and N-hydroxysuccinimide (to form active esters), mixed with cells and extruded into calcium

The reactive triazine dye, Cibacron Blue F3G-A, has been covalently attached to microparticulate porous silica and used for the resolution of a number of complementary proteins by high-performance liquid affinity chromatography (HPLAC). Cibacron Blue F3G-A was converted to its 6-aminohexyl derivative by reaction with 1,6-diaminohexane and coupled to γ-glycidoxypropyltrimethoxysilane-activated poro