The protonation status of key residues and bound ligands are often important for the function of a protein. Unfortunately, protons are not discerned in normal protein crystal structures, so their positions have to be determined by more indirect methods. We show that the recently developed quantum refinement method can be used to determine the position of protons in crystal structures. By replacing

Popular Abstract in Swedish Avhandlingen behandlar den kristna minoriteten Old Order Amish i USA ur ett ecklesiologiskt perspektiv. Genom att dels använda centrala begrepp som utgår från den undersökta gruppens egen religiösa vokabulär, dels några av de angreppssätt som är förhärskande inom det kyrkovetenskapliga studiet, framträder en fördjupad bild av amishfolkets insida. Hur uppfattar amish siThe thesis focuses on the way the Amish view their life and actions as Christians. With its basis in first-hand observations from the settlements of Dover (DE) and Lancaster (PA) the inquiry describes, analyzes and interprets the ecclesiastical life of the Old Order Amish from an ecclesiological viewpoint. Utilizing a specific model of analysis the dissertation examines the institutions (church/f

OBJECTIVE:To identify variables associated with rejection of synthetic, suburethral slings used for female stress urinary incontinence.METHODS:Between 1991 and 1998, gynecologists at our department performed 428 operations for stress urinary incontinence by inserting expanded polytetrafluoroethylene or polyethylene suburethral slings. After suitable exclusions, 386 women followed for at least 24 m

Popular Abstract in Swedish Många tolkningar och användningsområden har av olika forskare föreslagits för det gammaltestamentliga offret shelamin. Detta offer hade också en oklar plats i den israelitiska kulturen. Avsaknaden av konsensus och misslyckandet med att nå fram till en tillfredsställande lösning på gåtan gör shelamim till ett intressant offer att undersöka med hjälp av modern ritualforskFor the sacrifice shelamim in the Hebrew Bible, scholars have suggested a number of different interpretations and uses. This sacrifice also had an ambiguous role in Israelite culture. Lack of a general consensus and the failure to reach a satisfying solution makes the shelamim a puzzle and an interesting sacrifice to investigate with insights gained from modern ritual studies. In this study, it i

Resonance scattering of light as function of magnetic field has been used to investigate the hyperfine structure of the 4p2P3/2- and 5p2P3/2-terms in the potassiumI-spectrum, using a sample enriched in the K40-isotope. With increasing magnetic field strength a structure containing two characteristic signals for K40 was observed. Taking into account the small ratio of hyperfine structure splitting

Oxytocin receptor mimetics were prepared by molecular imprinting using Z-oxytocin as the template. Comparative binding studies with reference polymers showed that the imprinted polymers recognized both Z-oxytocin and unprotected oxytocin selectively. The dissociation constants were 47 µM and 102 µM,respectively, and the density of binding sites was 12 µmol/g. The synthetic oxytocin receptors were

Wound botulism is a growing problem among injecting drug users. The condition is often difficult to diagnose, with laboratory confirmation in only 50% of the cases. Here we present a real-time PCR-based method for the diagnosis of wound botulism caused by Clostridium botulinum. The assay includes an internal amplification control which is amplified simultaneously with the genes encoding neurotoxin

This article is based on an analysis of Lund University that took place during the summer and autumn of 2004 (available for download at www.iiiee.lu.se, click library and publications). The university had experienced a loss of momentum in their progress regarding environmental issues. The purpose of the study was to identify barriers to including sustainability-related content throughout Lund Univ

In colostrum collected 0-80 h postpartum the contents of immunoglobulins (Igs), transforming growth factor beta-2 (TGF-beta2), insulin-like growth factor-1 (IGF-1) and growth hormone (GH) were analysed. Colostrum initially contained 90 mg mL(-1) IgG1, 2.8 mg mL(-1) IgG2, 1.6 mg mL(-1) IgA, 4.5 mg mL(-1) IgM, and these concentrations declined by 92%, 87%, 93% and 84%, respectively, in the samples c

As part of the restoration of biodiversity on former agricultural land there has been focused on methods to enhance the rate of transition from agricultural land towards natural grasslands or forest ecosystems. Management practices such as sowing seed mixtures and inoculating soil of later successional stages have been used. The aim of this study was to determine the effects of a managed plant com

Early diagnosis of atherosclerosis is essential as many of the risk factors are life-style dependent. We suggest a new method to measure minute roughness of the size of micrometers of the arterial wall. The method was evaluated using three silicone phantoms sized 13 fun, 23 itm and 33 pm, respectively. The mean of the measured heights of the phantoms were 8.1 mu m (SD 0.0), 23.3 mu m (SD 0.2) and

Binding properties of staphylococcal protein A (SpA) to different human immunoglobulins have been investigated. In this analysis, intact SpA as well as SpA-derived fragments containing one to five IgG-binding domains of different compositions, were used. The affinity binding constants of the different proteins to human polyclonal IgG, IgA, IgM and F(ab')2-fragments as well as their binding capacit

The four-year visual outcome was retrospectively studied in patients with older-onset diabetes mellitus and diabetic retinopathy in need of laser treatment. Visual acuity in 53 patients examined by ophthalmologists who referred the patients for an evaluation of retinopathy before laser treatment, was compared to that of 47 patients examined by ophthalmologists who also performed the photocoagulati

BACKGROUND: Although abundant data are available regarding the prevalence of chronic hepatitis B or C virus (HBV, HCV) among both blood donors and patients with liver diseases, corresponding data for the general population are scarce. Accordingly, this study was designed to investigate the prevalence and clinical spectrum of HBV and HCV in a general Swedish middle-aged urban population. METHODS: D

Distributions of event-by-event fluctuations of the mean transverse momentum and mean transverse energy near mid-rapidity have been measured in Au+Au collisions at roots(NN)=130 GeV at the Relativistic Heavy-Ion Collider. By comparing the distributions to what is expected for statistically independent particle emission, the magnitude of nonstatistical fluctuations in mean transverse momentum is de