Determination of fatty acid composition in phospholipids using Supercritical Fluid Chromatography - Mass Spectrometry
Determining fatty acid composition within different lipid classes is a time-consuming process. Generally, normal phase chromatography is used to separate lipids by lipid class, based on differences in polarity. Fractions of this separation are then collected and derivatized into fatty acid methyl esters (FAMES), followed by analysis with gas chromatography and flame ionization detection (GC-FID).