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Estimation of in vivo pulmonary microvascular and interstitial geometry using digital image analysis

OBJECTIVE: To determine microvascular diameter and perivascular interstitium thickness at the lung surface in the in situ, in vivo lung. METHODS: Microscopic images of the lung surface collected through a "pleural window" by a videocamera were digitized with a monochrome frame grabber (512 x 512 pixels, 8 bits per pixels) to be computer analyzed by image processing techniques. RESULTS: We found th

Reed warbler orientation: initiation of nocturnal migratory flights in relation to visibility of celestial cues at dusk

We used radiotelemetry to investigate the time of migratory flight initiation relative to available celestial orientation cues and departure direction of a nocturnal passerine migrant, the reed warbler, Acrocephalus scirpaceus, during autumn migration. The study was carried out at Falsterbo, a coastal site in southwest Sweden. The warblers initiated migration from times well after local sunset and

The emotional climate of care-giving in home-care services

The emotional aspects of the care-giving relationship in home-care services are studied, starting from the home-care recipients' and the home-care workers' perception of the emotional climate. Their experiences of the care-giving relationship and the influence from different aspects of the care-giving situation and social processes in the work organisation are explored. Two hundred and twenty-two

Echoes of Pearl in Arda's Landscape

The world of Arda and the many stories set therein carry within them echoes of earlier tales, and even though it would be terribly reductive to discuss Tolkien’s work only in terms of its sources, knowledge of where the echoes come from contribute to our understanding and, perhaps more importantly, enjoyment of his world and stories. This is illustrated as much by the numerous university courses t

Seasonal and inter-annual photosynthetic response of representative C-4 species to soil water content and leaf nitrogen concentration across a tropical seasonal floodplain

We examined the seasonal and inter-annual variation of leaf-level photosynthetic characteristics of three C-4 perennial species, Cyperus articulatus, Panicum repens and Imperata cylindrica, and their response to environmental variables, to determine comparative physiological responses of plants representing particular microhabitats within a seasonal tropical floodplain in the Okavango River Delta,

Extended analysis of the TiI spectrum: 3d-4f, 4d-4f and 4f-5g transitions

Titanium spectra recorded in the infrared wavelength region with a Fourier Transform Spectrometer have been analysed, and 572 lines have been identified as transitions of the types 3d-4f, 4d-4f and 4-5g. 11 lines in the UV region around 2400 angstrom have been identified as belonging to the 3d(3)4s a(5)F-3d(2)4s(F-4)4f transition array. 113 levels of the 3d(2)4s(F-4)4f, 3d(3)(F-4)4f and 3d(2)4s(F-

Modulation of inflammatory mediators and ppargammaand nfkappab expression by pravastatin in response to lipoproteins in human monocytes in vitro.

Statins are inhibitors of the rate-limiting step of cellular cholesterol synthesis. In vitro and in vivo studies suggest that statins have anti-inflammatory properties independent of their cholesterol-lowering effects. These observations prompted us to examine the effects of pravastatin (50 mgr; M) and native or oxidized low density lipoprotein (nLDL or oxLDL) (50 mgr; g ml(minus sign1)) on primar

Oncoretroviral gene transfer to NOD/SCID repopulating cells using three different viral envelopes

Background The aim of this study was to investigate gene transfer to human umbilical cord blood (CB) CD34(+)/CD38(low) and NOD/SCID repopulating cells using oncoretroviral vectors and to compare the transduction efficiency using three different viral envelopes. Methods CB cells were transduced on Retronectin using an MSCV-based vector with the gene for GFP (MGIN), which was packaged into three dif

Adenovirus-mediated overexpression of murine cyclic nucleotide phosphodiesterase 3B

To construct the recombinant adenovirus vector containing the cDNA for recombinant mouse cyclic nucleotide phosphodiesterase 3B (mPDE3B), the cDNA for mPDE3B was subcloned into pACCMV.pLpA. Subsequently, this recombinant plasmid, pACCMV.mPDE3B, was cotransfected with pJM17 plasmid containing the adenoviral genome into 293 human embryonic kidney cells, and the replication-deficient adenovirus AdCMV

Extracytoplasmatic processes impaired by inactivation of the trxA (thioredoxin gene) in Bacillus subtilis

The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5'-phosphosulfate/3'-phosphoa

Challenges and opportunities for medications development in alcoholism: An international perspective on collaborations between academia and industry

This article represents the proceedings of a symposium presented at the 12th Congress of the International Society for Biomedical Research on Alcoholism held in Heidelberg/Mannheim, Germany, on September 30, 2004. The organizers and cochairs were Bankole A. Johnson, DSc, MD, PhD, and Karl Mann, MD. The presentations included the following: (1) A Perspective from Academia, by Bankole A. Johnson, DS

On the theory of low-density convolutional codes

We introduce and analyze a new statistical ensemble of lowdensity parity-check convolutional (LDC) codes. The result of the analysis are bounds, such as a lower bound for the free distance and upper bounds for the burst error probability of the LDC codes.

Pion and kaon electromagnetic form factors

We study the electromagnetic form factor of the pion and kaons at low-energies with the use of Chiral Perturbation Theory. The analysis is performed within the three flavour framework and at next-to-next-to-leading order. We explain carefully all the relevant consistency checks on the expressions, present full analytical results for the pion form factor and describe all the assumptions in the anal

Synthesis and reactivity studies of a manganese microperoxidase containing b-type heme

Mn(III) protoporphyrin IX-6(7)-gly-gly-his methyl ester (MnGGH) has been prepared by condensation of glycyl-glycyl-L-histidine methyl ester with the propionic side chains of Mn(III) protoporphyrin IX. It was characterised by mass spectrometry and UV/VIS spectroscopy. Stopped-flow spectrophotometry was used to study the reaction of the Mn microperoxidase with hydrogen peroxide. The formation of act

Volumetric intensity dependence on the formation of molecular and atomic ions within a high intensity laser focus

The mechanism of atomic and molecular ionization in intense, ultra-short laser fields is a subject which continues to receive considerable attention. An inherent difficulty with techniques involving the tight focus of a laser beam is the continuous distribution of intensities contained within the focus, which can vary over several orders of magnitude. The present study adopts time of flight mass s