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Reliable assessment of self-reported immune function is essential for identifying at-risk populations, monitoring public health interventions and informing global health strategies. Yet, current instruments are imprecise and lack cross-cultural validity. This study aimed to assess the cross-cultural validity of the 7-item Immune Status Questionnaire (ISQ). The Partial Credit Rasch model was applie
A large number of cities have created semantic 3D city models, but these models are rarely used as input data for simulations, such as noise and flooding, in the urban planning process. Reasons for this are that many simulations require detailed land cover (LC) and elevation data that are often not included in the 3D city models, and that there is no linkage between the elevation and land cover da
Children and adolescents with obsessive-compulsive disorder (OCD) are at increased risk of psychotic disorders; however, the manifestations and clinical implications of psychotic vulnerability among youth with OCD remain poorly understood. To address this gap, we examined the prevalence and clinical correlates of psychotic vulnerability in youth with OCD across two independent samples. Using the T
Posttraumatic stress disorder (PTSD) and obsessive-compulsive disorder (OCD) share ostensibly similar and sometimes overlapping symptoms that complicate diagnostic assessment and conceptualization. While varying models for the symptom structure of PTSD have been proposed – including the presently used 4-factor and a more fine-grained 7-factor model – little research has focused on youth and even l
Water is most important to all lives on this planet, but there is a lot of fresh water wasted on the land. With the rapid increase of population, the humankind shall pay more attention to water storage on the land. Inspired by the “sponge city” theory put forward by some researchers in urban construction in recent years, the authors try to put forward the “sponge earth” theory, so as to make enoug
As universities compete for higher standings in global rankings and implement performance management measures, performance pressure is imposed on scholars. This paper explores the influence of academic systems and career models on scholars in Sweden and Finland, with a focus on experienced performance pressure. Comparable qualitative and quantitative data from these Nordic countries were collected
OBJECTIVE: To explore the cerebrospinal fluid (CSF) proteome in systemic lupus erythematosus (SLE) and the associations between the CSF proteomic patterns and clinical manifestations.METHODS: CSF samples from 29 female outpatients with SLE were analyzed with label-free liquid chromatography tandem mass spectrometry. Inclusion and CSF collection were conducted irrespective of clinical manifestation
Portal hypertension (PH) is a major complication of chronic liver disease, often leading to serious clinical consequences such as variceal bleeding, ascites, and splenomegaly. The current gold standard for PH diagnosis, namely, hepatic venous pressure gradient measurement, is invasive and not widely available. Transient elastography has emerged as a non-invasive alternative for assessing liver sti
Hydroxylamine was used to cleave the Asn-Gly peptide bond between the fusion partner and the antimicrobial peptide of interest, a magainin derivative (MSI-344). The efficiency of reaction depended on the hydroxylamine concentration, denaturant, pH, and the fused protein concentration. The optimal cleavage solution consisted of guanidine ⋅ HCl as the denaturant, pH 8.1, and 6.7 mg ml−1 of fuse
Triton X-114 and cation-exchange chromatography, SP-Sepharose FF, removed endotoxins from solutions containing recombinant histone H1.5. Dissociated endotoxins were removed but fractions containing histone H1.5 were enhanced in the elution step. The final concentration of endotoxins, measured by a limulus amoebocyte lysate (LAL) assay, was below 0.05 EU mg−1 histone H1.5. The recovery of protein w
A magainin derivative, designated MSI-344, was produced in Escherichia coli as fusion protein, by utilizing a truncated amidophsphoribosyltransferase of E. coli as a fusion partner. Bacterial cells transformed with the gene encoding the fusion protein were grown to a high cell density and induced with isopropyl-1-thio-b-D-galatoside (IPTG) to initiate product expression. The fusion protein was a
An Escherichia coli expression system has been constructed for production of biologically active recombinant histone H1.5. A process of fermentation and purification method at a large scale has been developed. Recombinant histone H1.5 was released from the high density cultured cells by high-pressure homogenization. For an efficient removal of cell debris and partial purification of basic histone
Capillary leak syndrome is a serious postoperative complication in neonates and infants after open-heart surgery for CHD. The aim of this study was to investigate the relationship between sternal opening width and the occurrence of capillary leak syndrome in patients undergoing cardiac surgery with delayed sternal closure.We retrospectively analysed the clinical data of neonates and infants (aged
Förhållandet mellan staten och det vi idag kallar det civila samhället har under de senaste århundradena radikalt ändrat karaktär när det gäller organiseringen av religionsutövning
Disjunct distribution patterns have long intrigued biogeographers, sparking ongoing debates about the mechanisms driving the current distribution of biodiversity. Among the most discussed patterns are long-distance dispersal and vicariance. While these patterns have been extensively studied in plants, marine taxa, mammals, and some invertebrates, they remain less explored in groups like moths. In
Electrodialysis is a water desalination technology that enables selective separation of ions, making it a promising solution for sustainable water reuse. The selectivity of the process is mainly determined by the properties of ion exchange membranes that can vary depending on the composition of ions in water, such as water uptake and charge density. In this work, we studied selective adsorpti
This article presents findings from a quantitative analysis of 215 case files regarding incidents of the crime of sexual molestation reported to the Swedish Police. We track the incidents through the criminal justice system and show a clearance rate of 9%. The clearance rate is higher when the attack is of a physical nature, the suspect is a stranger, and the incident occurs in a public place. Whi
