The cDNA of the human wild-type p53 tumor suppressor gene was constitutively overexpressed in the leukemic cell line K562 (which lacks detectable amounts of p53 protein) in order to investigate the consequences for growth and differentiation. Several stable clones were established by transfection of the expression vector pc53SN3. Expression of p53 protein was characterized by biosynthetic labeling

Maternal stress during gestation has the potential to affect offspring development via changes in maternal physiology, such as increases in circulating levels of glucocorticoid hormones that are typical after exposure to a stressor. While the effects of elevated maternal glucocorticoids on offspring phenotype (i.e., “glucocorticoid-mediated maternal effects”) have been relatively well established

Extension of the substrate range is among one of the metabolic engineering goals for microorganisms used in biotechnological processes because it enables the use of a wide range of raw materials as substrates. One of the most prominent examples is the engineering of baker’s yeast Saccharomyces cerevisiae for the utilization of D-xylose, a five-carbon sugar found in high abundance in lignocellulosi

Recent theoretical explanations for how hydrodynamic-like flow can build up quickly in small collision systems (hydrodynamization) has led to a microscopic picture of flow building up in a gluon-dominated phase before chemical equilibrium between quarks and gluons has been attained. The goal of this contribution to Offshell-2021 is to explore consequence of assuming a long-lived gluon-dominated ph

Parental influence over children’s status attainment has historically been argued to be key. However, the cross-sibling influence has been scarcely studied for historical periods and for steam family societies, being the most long-lasting relationship across individuals’ lives once childhood was surpassed. We investigate how intra-generational family relationships determine the social destiny of s

This chapter aims to explore community energy (CE)—energy communities that are established and run by private citizens—in four Eastern European countries. The chapter first analyses general contextual conditions supporting the diffusion of CE initiatives in the Baltic Sea Region. Subsequently, it reports the findings of four case studies from Estonia, Latvia, Lithuania, and Poland illustrating som

Objectives Emerging evidence supports that PR interval prolongation is associated with increased mortality. However, most previous studies have limited confounder control, and clinical impact in a population of acute ill patients is unknown. The aim of this study was to investigate whether 1-year all-cause mortality was increased in patients presenting with PR interval prolongation in the emergenc

Objectives: The aim of this study was to assess the incidence of infection after transrectal prostate biopsy (TRbx). Secondary objectives were to describe infection characteristics, antibiotic resistance patterns, ICD-10 coding, and costs. Methods: TRbx carried out at the hospitals of Ängelholm and Helsingborg, Scania, Sweden, between October 2017 and March 2019, were identified based on the NOMES

Snow is one of the essential factors in hydrology, freshwater resources, irrigation, travel, pastimes, floods, avalanches, and vegetation. In this study, the snow cover of the northern and southern slopes of Alborz Mountains in Iran was investigated by considering two issues: (1) Estimating the snow cover area and the (2) effects of droughts on snow cover. The snow cover data were monitored by ima

Air temperature is a vital meteorological variable required in many applications, such as agricultural and soil sciences, meteorological and climatological studies, etc. Given the importance of this variable, this study seeks to estimate minimum (Tmin), maximum (Tmax), and mean (T) air temperatures by applying a linear autoregressive (AR) time series model and then developing a hybrid model by mea

Phimosis in boys is a common condition. In the majority of cases it is physiological without symptoms. Symptomatic phimosis can often be treated successfully with local steroid cream - only a minority of patients need surgery. This study concludes that diagnosis, information to patients and guardians, initiation and follow-up of local steroid treatment can successfully, and safely, be delegated to

The market for partly or fully electrified vehicles is expanding fast. The number of sources and loads that are connected to the vehicles traction voltage systems (TVS) increase and thus also the EMC requirements on these sources and loads. These requirements should make sure that neither function nor lifetime of any source or load is severely affected by another. The EMC requirements include both

This study compared second languagevocabulary acquisition from engagement with two different online media: written blog posts and video blogs. It also explored whether there were differences betweenwhich aspects of vocabulary knowledge(i.e., orthography, semantics, and grammatical function) were best learned from these media. The results showed that incidental vocabulary learning occurred in appro

We have performed an exclusive measurement of the K-+He3→Λpn reaction at an incident kaon momentum of 1GeV/c. In the Λp invariant mass spectrum, a clear peak was observed below the mass threshold of K̄+N+N, as a signal of the kaonic nuclear bound state, K̄NN. The binding energy, decay width, and S-wave Gaussian reaction form factor of this state were observed to be BK=42±3(stat.)-4+3(syst.)MeV, ΓK

Peatlands and forests cover large areas of the boreal biome and are critical for global climate regulation. They also regulate regional climate through heat and water vapour exchange with the atmosphere. Understanding how land-atmosphere interactions in peatlands differ from forests may therefore be crucial for modelling boreal climate system dynamics and for assessing climate benefits of peatland