RATIONALE: Tandem mass (MS/MS) spectra generated by collision-induced dissociation (CID) typically lack redundant peptide sequence information in the form of e.g. b- and y-ion series due to frequent use of sequence-specific endopeptidases cleaving C- or N-terminal to Arg or Lys residues.METHODS: Here we introduce arginyl-tRNA protein transferase (ATE, EC 2.3.2.8) for proteomics. ATE recognizes aci

Gram-positive bacterium Streptococcus mutans is the primary causative agent of human dental caries. To better understand this pathogen at the atomic structure level and to establish potential drug and vaccine targets, we have carried out structural genomics research since 2005. To achieve the goal, we have developed various in-house automation systems including novel high-throughput crystallizatio

In computer vision, many problems can be formulated as finding a low rank approximation of a given matrix. Ideally, if all elements of the measurement matrix are available, this is easily solved in the L2-norm using factorization. However, in practice this is rarely the case. Lately, this problem has been addressed using different approaches, one is to replace the rank term by the convex nuclear no

Objectives We recently constructed a general population job exposure matrix (JEM), The Shoulder JEM, based on expert ratings. The overall aim of this study was to convert expert-rated job exposures for upper arm elevation and repetitive shoulder movements to measurement scales. Methods The Shoulder JEM covers all Danish occupational titles, divided into 172 job groups. For 36 of these job groups,

Monocytes and macrophages are part of the body's first line of defence, eliminating pathogens by phagocytosis or by releasing a broad array of inflammatory mediators, such as cytokines, chemokines, and proteases. In humans, 3 subsets of monocytes are described in blood with seemingly different functions, the classical (CD14 + + CD16 -) monocytes, the intermediate (CD14 + + CD16 +) monocytes, and t

Purpose - The purpose of this paper is to investigate the numerical fluid-structure interaction (FSI) framework for the simulations of mechanical behavior of new vortex generators (VGs) in smooth wavy fin-and-elliptical tube (SWFET) heat exchanger using the ANSYS MFX Multi-field® solver. Design/methodology/approach - A three-dimensional FSI approach is proposed in this paper to provide better unde

P-type ATPases from the P4 subfamily (P4-ATPases) are energy-dependent transporters, which are thought to establish lipid asymmetry in eukaryotic cell membranes. Together with their Cdc50 accessory subunits, P4-ATPases couple ATP hydrolysis to lipid transport from the exoplasmic to the cytoplasmic leaflet of plasma membranes, late Golgi membranes, and endosomes. To gain insights into the structure

Background: In a global perspective, bacterial infections are still a major cause of morbidity and mortality; therefore, effective antibiotics are needed. However, the emergence of antibiotic resistance due to irrational use has now become a serious public health problem. Hence, the objective of this study was to analyse the association of regulatory aspects with antibiotic consumption. Methods: A

Falsterbo Peninsula on the south coast of Sweden is low-lying and exposed to flooding. In 1872 the extremestorm surge Backafloden caused large devastation on the Swedish, Danish, and German coasts in the SouthBaltic Sea. For the Falsterbo Peninsula, the peak storm surge level is estimated to have been 240 cm abovenormal. If a similar event happened today, the consequences would be far worse, as exFalsterbo Peninsula on the south coast of Sweden is low-lying and exposed to flooding. In 1872 the extreme storm surge Backafloden caused large devastation on the Swedish, Danish, and German coasts in the South Baltic Sea. For the Falsterbo Peninsula, the peak storm surge level is estimated to have been 240 cm above normal. If a similar event happened today, the consequences would be far worse, as

Inherited germline polymorphisms can cause gene expression levels in normal tissues to differ substantially between individuals. We present an analysis of the genetic architecture of normal adult skin from 470 genetically unique mice, demonstrating the effect of germline variants, skin tissue location, and perturbation by exogenous inflammation or tumorigenesis on gene signaling pathways. Gene net

This paper is about interdisciplinary common initiatives to enhance and encourage quality of engineering education nationwide, by means of a scientific approach to teaching. To reach innovative pedagogical approaches it is seen as important to stimulate collaboration and knowledge sharing across discipline borders. STEM, referring to the academic disciplines of science, technology, engineering, an

Background: The gut microbiota plays an important role in the development of obesity and obesity-associated impairments such as low-grade inflammation. Lingonberries have been shown to prevent diet-induced obesity and low-grade inflammation. However, it is not known whether the effect of lingonberry supplementation is related to modifications of the gut microbiota. The aim of the present study was

Study objective: We evaluate the diagnostic accuracy of a high-sensitivity cardiac troponin T (hs-cTnT) level less than 5 ng/L or less than or equal to 14 ng/L at emergency department (ED) presentation, combined with the emergency physician's assessment of history and ECG, for ruling out major adverse cardiac events within 30 days. Methods: This prospective observational study enrolled consecutive