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Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography

Trichoderma cellulases appear in several isoforms which makes their purification and analysis difficult. We used fast protein liquid chromatography (FPLC) to purify three major cellulases and to quantitate these enzymes in reconstituted mixtures during cellulose hydrolysis studies (in lack of specific substrates and because of the synergism between the enzymes such analysis is very difficult, if a

Purification and characterisation of a water-soluble ferrochelatase from Bacillus subtilis

Bacillus subtilis ferrochelatase is encoded by the hemH gene of the hemEHY gene cluster and catalyses the incorporation of Fe2+ into protoporphyrin IX. B. subtilis ferrochelatase produced in Escherichia coli was purified. It was found to be a monomeric, water-soluble enzyme of molecular mass 35 kDa which in addition to Fe2+ can incorporate Zn2+ and Cu2+ into protoporphyrin IX. Chemical modificatio

Canavanine content of sword beans (Canavalia gladiata) – analysis and effect of processing

The amino acid canavanine is a potentially toxic constituent of leguminous seeds. The aim of the present study was to determine the ability of different processing methods to reduce canavanine in sword beans (Canavalia gladiata). For this purpose a method for the detection and quantification of canavanine was developed using reversed-phase high-performance liquid chromatography of the dabsylated d

Quantum mechanical wavepacket transport in quantum cascade laser structures

We present a viewpoint of the transport process in quantum cascade laser structures in which spatial transport of charge through the structure is a property of coherent quantum mechanical wave functions. In contrast, scattering processes redistribute particles in energy and momentum but do not directly cause spatial motion of charge.

Influence of urban geometry on outdoor thermal comfort in a hot dry climate: A study in Fez, Morocco

There are few studies on the microclimate and human comfort of urban areas in hot dry climates. This study investigates the influence of urban geometry on outdoor thermal comfort by comparing an extremely deep and a shallow street canyon in Fez, Morocco. Continuous measurements during the hot summer and cool winter seasons show that, by day, the deep canyon was considerably cooler than the shallow

A four-year follow-up study on the incidence of diabetic retinopathy in older onset diabetes mellitus

Out of 369 diabetic patients with an age at onset of diabetes > or = 30 years previously studied, 325 (88%) were included in an ophthalmological follow-up examination 4 years later. In patients treated with oral drugs at baseline, the incidence of any type of retinopathy was 30.8% and of severe retinopathy 5.7%. All patients who developed severe retinopathy received insulin during the follow-up pe

Leaving Home in a Peasant Society. Economic Fluctuations, Household Dynamics and Youth Migration in Southern Sweden, 1829-1866.

Popular Abstract in Swedish Avhandlingen behandlar barns flyttningar hemifrån i det förindustriella bondesamhället. Utöver att studera när barn lämnade hemmet kopplas också flyttningsprocessen till den förindustriella hushållsekonomins funktionssätt. Analysen görs på ett longitudinellt material för ett område i västra Skåne under perioden 1829-1866. Individerna följs från födseln, eller från det aThis study deals with the process of children leaving home and its interactions with the household economy in preindustrial society. A theoretical model of leaving home is outlined and then analyzed multivariately, using a longitudinal micro-level dataset for a sample of parishes in western Scania, Sweden, for the period 1829-1866. In a first introductory part the social and economic background as

Greater reduction of knee than hip pain in osteoarthritis treated with naproxen, as evaluated by WOMAC and SF-36

Objectives: To compare the improvement of hip and knee osteoarthritis during treatment with naproxen. Methods: Men and women aged 40 to 75 years with symptomatic osteoarthritis of the knee or hip of at least three months' duration participated in a six week placebo controlled, double blind study with naproxen 500 mg twice daily as one treatment arm. Naproxen was given to 403 patients (280 knee, 12

Submaximal-exercise-induced impairment of human muscle to develop and maintain force at low frequencies of electrical stimulation

The aim of this study was to test the hypothesis that low intensity exercise-induced low frequency fatigue is caused by failure of excitation-contraction coupling. Changes in knee extension torque at 5, 10, 15, 20 and 50 Hz electrical stimulation of quadriceps muscle in ten healthy, young, male subjects were recorded during 20-min voluntary exercise followed by 60-min recovery. In seven of the ten

Woven convolutional codes II: Decoding aspects

An iterative decoding scheme for woven convolutional codes is presented. It operates in a window sliding over the received sequence. This exploits the nature of convolutional codewords as infinite sequences and reflects the concept of considering convolutional encoding and decoding as a continuous process. The decoder is analyzed in terms of decoding delay and decoding complexity. Its basic buildi