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An Ael allele-specific nucleotide insertion at the blood group ABO locus and its detection using a sequence-specific polymerase chain reaction

Genomic DNA from each of four Acl individuals (genotypes AO1, AO1var, AO2) and one AclB individual was used as a template for amplifying exons 6 and 7 of the ABO genes, which were subsequently sequenced. In all the Ael alleles a single nucleotide insertion, compared to the A consensus sequence, was observed that would alter the amino acid sequence of the glycosyltransferase immediately after its p

Peripheral effects of PTH are not altered after thyroid surgery in euthyroid patients

Background. We have previously found decreased serum levels of both ionized calcium and 1,25(OH)(2)D and an increase in serum phosphate levels at 1 year after hemithyroidectomy. However, basal and stimulated parathyroid hormone (PTH) secretions were not altered. To investigate whether the observed biochemical changes after unilateral thyroid surgery may be due to a relative end-organ resistance to

Human hemoglobin adducts following exposure to hexahydrophthalic anhydride and methylhexahydrophthalic anhydride

Hexahydrophthalic anhydride (HHPA) and methylhexahydrophthalic anhydride (MHHPA) are highly allergenic compounds used in the chemical industry. The aim of this study was to characterize the protein adducts in erythrocytes following exposure to HHPA and MHHPA. Blood and urine samples were obtained from 51 HHPA- and MHHPA-exposed workers. Erythrocytic proteins from HHPA- and MHHPA-exposed workers we

Growth and capture activities of nematophagous fungi in soil visualized by low temperature scanning electron microscopy

Growth and capture activities of nematophagous fungi in soil were observed using the low temperature scanning electron microscopy technique. The advantages of this sample preparation technique, compared with conventional scanning electron microscopy, are that it does not involve chemical fixation and dehydration of the sample and, therefore, gives a more accurate view of organ isms in soil. Growth

Spatial and temporal changes in South African solar ultraviolet-B exposure: Implications for threatened taxa

Spatial and temporal changes in South African solar UV-B (280-315 nm) exposure were modeled and mapped at 2.5 km2 resolution using ozone, relative humidity, cloud amount and elevation data. Computations indicated large natural gradients in UV-B exposure ranging from a 34.2% change over 14o of latitude to a 44.2% change over 16o of longitude. Modelling of future scenarios in annual UV-B exposure in

Interaction between localized and extended modes of oxygen in silicon

The interaction between localized and extended vibrational modes in solids is of central importance in understanding how local vibrational modes (LVMs) decay into phonons. In this study, we have investigated interstitial oxygen (O-i) in silicon as a model 'laboratory' for such local-extended mode interactions. Using hydrostatic pressure and infrared spectroscopy, we brought the stretch mode of O-1

The Pd (100)-(root 5 x root 5)R27 degrees-O surface oxide: A LEED, DFT and STM study

Using low energy electron diffraction (LEED), density functional theory (DFT) and scanning tunneling microscopy (STM), we have re-analyzed the Pd(100)-(root 5 x root 5)R27 degrees-O surface oxide structure consisting, in the most recent model, of a strained PdO(101) layer on top of the Pd(100) surface [M. Todorova et al., Surf. Sci. 541 (2003) 101]. Both, DFT simulations using the Vienna Ab initio

Bed agglomeration problems in fluidized-bed biomass combustion

Bed material agglomeration was studied experimentally in a fluidized-bed biomass combustor, Four biomass fuels, four bed materials, three bed additives, and three NOx additives were tested in a temperature range of 670-870 degreesC and at two pressure levels of 1.0 and 1.5 MPa. Two types of agglomeration were observed, a homogeneous and a heterogeneous type. The first occurred at low temperature a

Profibrotic effects of endothelin-1 via the ETA receptor in cultured human cardiac fibroblasts

Background/Aims: Endothelin-1 (ET-1) has been implicated in pathologic remodelling and tissue repair processes in the heart. We investigated the effects of ET-1 on growth and collagen synthesis responses in cardiac fibroblasts isolated from human hearts. We also studied the receptor subtype(s) mediating such responses and the factors regulating their expression. Methods: Fibroblasts were isolated

The early Nordic software effort

Early Nordic cooperation comprised ideas, programs, even computer blueprints and resulted in rapid progress. Regnecentralen contributed importantly in developing Algol 60. Algol for Facit EDB was used at many sites. Further advances were Datasaab's Algol Genius and the Norwegian Simula project. Regrettably, failure of our compiler companies led to software setbacks. We revisit the Algol debates co

Total column densities of tropospheric and stratospheric trace gases in the undisturbed Arctic summer atmosphere

Ground-based FTIR measurements have been performed in the Arctic summer in July 1993 and June 1994 at 79 degrees N to study the zenith column densities of several trace gases in the undisturbed Arctic summer atmosphere. Zenith column densities of H2O, N2O, HNO3, NO2, NO, ClONO2, ClO, HCl, HF, COF2, OCS, SF6, HCN, CH4, C2H6, C2H2, CO, O-3, CFC-12, CFC-22, and CO2 were retrieved by line-by-line calc

The influence of dietary nucleotides on erythrocyte membrane fatty acids and plasma lipids in preterm infants

OBJECTIVE: The objective of this study was to evaluate whether a regular formula for premature infants supplemented with nucleotides has any influence on plasma lipids and erythrocyte membrane fatty acids. METHODS: Preterm infants fed either human milk supplemented with human milk protein (HM, n = 14), nucleotide-supplemented preterm formula (NF, n = 13), or a regular preterm formula (F, n = 13) w