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SNARE-proteins (soluble NSF-attachment protein receptor) are important for Ca2+-dependent exocytosis. We have used capacitance measurements and confocal imaging to dissect the role of synaptosomal protein of 25 kDa (SNAP-25) and syntaxin 1 in rapid exocytosis in insulin-secreting pancreatic ß-cells. Following immunoneutralization of syntaxin 1 and SNAP-25, exocytosis was strongly reduced and assoc

The electric dipole polarizability and first and second hyperpolarizabilities of HCl are computed within coupled cluster response theory. A systematic basis set convergence study with Dunning's correlation consistent basis sets is first performed. Correlation effects are studied using the coupled cluster singles and doubles and CC3 models. The latter takes iteratively into account the effect of co

A 25-year-old, with type I Diabetes Mellitus with a previous diagnosis of Protamine Allergy but not to human Insulin, started to notice anaphylactic reactions immediately after bolus with Insulin. Skin prick and intradermal test were positive to all insulins. Skin tests to other potential allergens resulted negative. Examination after bolus of Human Insulin revealed urticaria. Daily insulin requir

The 222Rn concentration simulated by the regional atmospheric modelREMOover Europe and western Siberia is compared to in-situ records in Europe, and discussed in the context of site effects for stations that are also part of a CO2 observing network. The REMO model has a limited spatial domain, forced at its lateral boundaries with meteorological fields of the European Centre for Medium-RangeWeathe

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Ultracentrifugation was used as a non-destructive method to separate dough into liquid, gel, gluten, starch and bottom phases. The protein composition in the different phases was investigated for dough prepared from spring wheat (Triticum aestivum L.). The SDS-PAGE, SE-HPLC and RP-HPLC methods were used for the analysis. The wheat protein composition of the liquid and gel phases consisted of album

Popular Abstract in Swedish Det är normalt, och oftast inget man reflekterar över, att en vävnadsskada (exempelvis ett hudsår) är förknippad med förhöjd smärtkänslighet. Denna delas in i två fenomen: hyperalgesi, en förhöjd upplevelse av smärtsamma stimuli, och allodyni, som innebär att ett vanligtvis milt stimulus, exempelvis lätt beröring, upplevs som smärtsamt. Hyperalgesi och allodyni är normaAn intense, potentially tissue-damaging (noxious) stimulus may lead to enhanced pain sensation of subsequent noxious stimuli (hyperalgesia) and to the perceiving of innocuous stimuli as painful (allodynia). This is thought to result to a large extent from strengthening of synapses established by primary afferent fibers onto spinal cord dorsal horn neurons. Here we investigated, using the postembed

The output factor for a therapy photon beam is defined as the dose per monitor unit relative to the dose per monitor unit in a reference field. Convolution models for photon dose calculations yield the dose in units normalized to the incident energy fluence with phantom scatter intrinsically modeled. Output factors calculated with the convolution method as the dose per unit energy fluence relative

The thermal unfolding of Amy 34, a recombinant alpha-amylase from Bacillus halodurans, has been investigated using differential scanning calorimetry (DSC). The denaturation of Amy 34 involves irreversible processes with an apparent denaturation temperature (T-m) of 70.8 degrees C at PH 9.0, with four transitions, as determined using multiple Gaussian curves. The T-m increased by 5 degrees C in the

An experimental study of the lower fp-shell nuclei Sc-42,Sc-43 was performed via alpha pn and alpha p evaporation, respectively, from Ne-20 + Si-28 and Mg-24 + Mg-24 fusion-evaporation reactions. The experiments were conducted with the Gammasphere and Microball detector arrays. The level schemes of both nuclei have been extended considerably. Terminating states associated with the f(7/2)(n) and d(

Background and Purpose: Currently, only 4.4% of the forested area in Croatia has been mapped in terms of the critical load of acidity, with exceedances reported for a couple of sites in the region of Gorski Kotar However, some sensitive areas such as the eastern part of Croatia, have not yet been mapped and as a result the exceedance of the critical load of acidity could be much larger than the on

Analys av Victor Sjöströms berättarteknik i långfilmen Ingeborg Holm i utvecklingen av den moderna spelfilm.