Cell-specific chemotyping and multivariate imaging by combined FT-IR microspectroscopy and orthogonal projections to latent structures (OPLS) analysis reveals the chemical landscape of secondary xylem
P>Fourier-transform infrared (FT-IR) spectroscopy combined with microscopy enables chemical information to be acquired from native plant cell walls with high spatial resolution. Combined with a 64 x 64 focal plane array (FPA) detector, 4096 spectra can be simultaneously obtained from a 0.3 x 0.3 mm image; each spectrum represents a compositional and structural 'fingerprint' of all cell wall compon