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Epithelial synthesis of tenascin at tips of growing bronchi and graded accumulation in basement membrane and mesenchyme

The extracellular matrix protein, tenascin, has been proposed as mediator in epithelial-mesenchymal interactions because of its characteristic distribution during embryogenesis. Here we compared the accumulation of tenascin and laminin in the early chicken lung bud. Laminin is deposited in the basement membrane, starting at the tips and increasing along the shafts of growing primary and secondary

Spray layer-by-layer films based on phospholipid vesicles aiming sensing application via e-tongue system

The Layer-by-Layer (LbL) technique via spraying (spray-LbL) has been applied as new and alternative methodology to fabricate ultrathin films due to its versatility in relation to the conventional dipping-LbL method, mainly in terms of faster layer deposition and larger coated area. In this work, the possibility of immobilizing vesicles of dipalmitoyl phosphatidyl glycerol (DPPG) phospholipid onto

Physical vapor deposited thin films of lignins extracted from sugar cane bagasse : Morphology, electrical properties, and sensing applications

The concern related to the environmental degradation and to the exhaustion of natural resources has induced the research on biodegradable materials obtained from renewable sources, which involves fundamental properties and general application. In this context, we have fabricated thin films of lignins, which were extracted from sugar cane bagasse via modified organosolv process using ethanol as org

Study of the interaction between cardiolipin bilayers and methylene blue in polymer-based Layer-by-Layer and Langmuir films applied as membrane mimetic systems

An increase of the reports involving mimetic systems has been observed. Briefly, these systems use biological phospholipids to exploit specific interactions between membrane-models and drugs. Here, the Layer-by-Layer (LbL) and Langmuir techniques were used to investigate the interaction between cardiolipin (CLP - negative phospholipid) and a cationic-like drug methylene blue (MB). Supported by a c

Isolation and characterization of a resident tolerant Saccharomyces cerevisiae strain from a spent sulfite liquor fermentation plant

Spent Sulfite Liquor (SSL) from wood pulping facilities is a sugar rich effluent that can be used as feedstock for ethanol production. However, depending on the pulping process conditions, the release of monosaccharides also generates a range of compounds that negatively affect microbial fermentation. In the present study, we investigated whether endogenous yeasts in SSL-based ethanol plant could

Structural and electric-optical properties of zinc phthalocyanine evaporated thin films : Temperature and thickness effects

It is known that the molecular architecture plays a fundamental role in the electrical and optical properties of materials processed in the form of thin films. Here, zinc phthalocyanine (ZnPc) thin films were fabricated through the vacuum thermal evaporation technique (PVD, physical vapor deposition) up to 50 nm thickness with the objective of determining their molecular architecture and some elec

Layer-by-layer technique as a new approach to produce nanostructured films containing phospholipids as transducers in sensing applications

Phospholipids are widely used as mimetic systems to exploit interactions involving biological membranes and pharmacological drugs. In this work, the layer-by-layer (LbL) technique was used as a new approach to produce multilayered thin films containing biological phospholipids applied as transducers onto Pt interdigitated electrodes forming sensing units of an electronic tongue system. Low concent

Exploiting distinct molecular architectures of ultrathin films made with iron phthalocyanine for sensing

The possibility of generating distinct film properties from the same material is crucial for a number of applications, which can only be achieved by controlling the molecular architecture. In this paper we demonstrate as a proof-of-principle that ultrathin films produced from iron phthalocyanine (FePc) may be used to detect trace amounts of copper ions in water, where advantage was taken of the cr

Sensor array made with nanostructured films to detect a phenothiazine compound

The detection of trace amounts of phenothiazines with fast, direct methods is important for medical applications and the pharmaceutical industry. In this paper we explore the concept of an electronic tongue to detect methylene blue (MB), with a sensor array comprising 6 units. These units were a bare Pt electrode, and Pt electrodes coated with 1 -layer LB films of dipalmitoyl phosphatidylcholine (

Molecular and morphological characterization of bis benzimidazo perylene films and surface-enhanced phenomena

Thin solid films of bis benzimidazo perylene (AzoPTCD) were fabricated using physical vapor deposition (PVD) technique. Thermal stability and integrity of the AzoPTCD PVD films during the fabrication (∼400 °C at 10 -6 Torr) were monitored by Raman scattering. Complementary thermogravimetric results showed that thermal degradation of AzoPTCD occurs at 675 °C. The growth of the PVD films was establi

Kinetics of photoinduced birefringence in the guest-host system of poly(methyl methacrylate) doped with azobenzene-containing crown ethers

The kinetics of the buildup and decay of photoinduced birefringence was examined in a series of host-guest systems: azobenzene-containing crown ethers, differing in the size of the crowns, dissolved in a poly (methyl methacrylate) matrix. In all samples, the kinetics of the buildup of the birefringence was reasonably described by a sum of two exponential functions, the time constants being inverse

A comparison of DSM-IV-TR and DSM-5 diagnostic criteria for gambling disorder in a large clinical sample

Background and aims: Gambling-related crimes are known to be associated with gambling disorder (GD). Due to a lack of consensus in the scientific community regarding the relevance of this diagnostic criterion, it was removed from the DSM-5. The primary aim of this study was to investigate through structural equation modeling (SEM) whether higher GD severity in treatment-seeking GD patients with a

Unreported exclusion and sampling bias in interpretation of randomized controlled trials in patients with STEMI

Aims: To assess the impact of sampling bias due to reported as well as unreported exclusion of the target population in a multi-center randomized controlled trial (RCT)of ST-elevation myocardial infarction (STEMI). Methods and Results: We compared clinical characteristics and mortality between participants in the DANAMI-3 trial to contemporary non-participants with STEMI using unselected registrie

Bioreduction

Bioreduction has emerged over the years as an alternative method to organic synthesis for the generation of chiral precursors of commercial interest. Bioreductions operate under mild conditions of pH and temperature with the help of highly regio- and enantio-selective oxidoreductase enzymes.In this contribution, the different oxidoreductase families involved in bioreductions are exemplified and th