On Monomeric and Trimeric dUTPases. Recombinant Expression, Purification, Conformational Properties and Catalytic Characteristics.
Nucleotide metabolism and replication of nucleic acids are processes of fundamental importance. A crucial enzyme in nucleotide metabolism is dUTPase (E.C. 3.6.1.23), that catalyses the hydrolysis of dUTP to dUMP and pyrophosphate. The activity of the enzyme has influence on the outcome of DNA replication and complete loss of enzyme activity is lethal, while a decreased activity promotes increased