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Anthocyanins and volatile phenylpropenes (isoeugenol and eugenol) in petunia (Petunia hybrida) flowers have the precursor 4-coumaryl coenzyme A (CoA) in common. These phenolics are produced at different stages during flower development. Anthocyanins are synthesized during early stages of flower development and sequestered in vacuoles during the lifespan of the flowers. The production of isoeugenol
Plants make specialized bioactive metabolites to defend themselves against attackers. The conserved control mechanisms are based on transcriptional activation of the respective plant species-specific biosynthetic pathways by the phytohormone jasmonate. Knowledge of the transcription factors involved, particularly in terpenoid biosynthesis, remains fragmentary. By transcriptome analysis and functio
Catharanthus roseus, the Madagascar periwinkle, synthesizes bioactive monoterpenoid indole alkaloids, including the anti-cancer drugs vinblastine and vincristine. The monoterpenoid branch of the alkaloid pathway leads to the secoiridoid secologanin and involves the enzyme iridoid synthase (IS), a member of the progesterone 5β-reductase (P5βR) family. IS reduces 8-oxogeranial to iridodial. Through
The medicinal plant Madagascar periwinkle (Catharanthus roseus) synthesizes numerous terpenoid indole alkaloids (TIAs), such as the anticancer drugs vinblastine and vincristine. The TIA pathway operates in a complex metabolic network that steers plant growth and survival. Pathway databases and metabolic networks reconstructed from 'omics' sequence data can help to discover missing enzymes, study m
Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is a complex and coordinate cellular process executed by petal limb cells of a Petunia×hybrida cv. 'Mitchell Diploid' (MD) plant. In MD flowers, the majority of benzenoid volatile compounds are derived from a core phenylpropanoid pathway intermediate by a coenzyme A (CoA) dependent, β-oxidative scheme. Metabolic flux analysis, reverse g
The emission of floral volatiles requires coordinated expression of biosynthetic genes. In the regulatory network of the volatile benzenoid/phenylpropanoid pathway in Petunia hybrida two master regulators of the pathway have been identified. The R2R3-MYB transcription factor EMISSION OF BENZENOIDS II (EOBII) utilizes a specific MYB binding site to activate the expression of the R2R3-MYB ODORANT1 (
In which cells of the flower volatile biosynthesis takes place is unclear. In rose and snapdragon, some enzymes of the volatile phenylpropanoid/benzenoid pathway have been shown to be present in the epidermal cells of petals. It is therefore generally believed that the production of these compounds occurs in these cells. However, whether the entire pathway is active in these cells and whether it i
Fragrance production in petunia flowers is highly regulated. Two transcription factors, ODORANT1 (ODO1) and EMISSION OF BENZENOIDS II (EOBII) have recently been identified as regulators of the volatile benzenoid/phenylpropanoid pathway in petals. Unlike the non-fragrant Petunia hybrida cultivar R27, the fragrant cultivar Mitchell highly expresses ODO1. Using stable reporter lines, we identified th
The exact biosynthetic pathways leading to benzoic acid (BA) formation in plants are not known, but labeling experiments indicate the contribution of both beta-oxidative and non-beta-oxidative pathways. In Petunia hybrida BA is a key precursor for the production of volatile benzenoids by its flowers. Using functional genomics, we identified a 3-ketoacyl-CoA thiolase, PhKAT1, which is involved in t
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The aim of this article is to study how selected variables influence delays in train traffic. Data has been collected on train movements, timetables, weather and capacity utilization on a highly utilized single-track railway line in southern Sweden during 2014. Based on this dataset, we have analysed how different factors affect delays in passenger traffic. We measure delays in a novel way, as devThe aim of this article is to study how selected variables influence delays in train traffic. Data has been collected on train movements, timetables, weather and capacity utilization on a highly utilized single-track railway line in southern Sweden during 2014. Based on this dataset, we have analysed how different factors affect delays in passenger traffic. We measure delays in a novel way, as dev
Purpose – The purpose of this paper is to gain new knowledge of how organizational errors can be used to early detect signals of impending crises and thereby develop internal crisis communication. Three communication processes – organizational culture, leadership and learning – that are particularly important for the development of internal crisis communication are focused. The paper also discusse
Punctuality is regarded as an important measure of the performance of a railway system, and is the one most commonly used and discussed measureboth in the industry and among travelers. In many countries,the punctuality of trains, and thus the performance of the railway system, is deemed as lacking. The aim of this article is to study and quantify how severalweather-,timetable, operational and infrPunctuality is regarded as an important measure of the performance of a railway system, and is the one most commonly used and discussed measure both in the industry and among travelers. In many countries, the punctuality of trains, and thus the performance of the railway system, is deemed as lacking. The aim of this article is to study and quantify how several weather-, timetable, operational and
Punctuality in Sweden has been too low for several years, and there is now a goal in the industry that it must improve by more than five percentage points by 2020. Research has shown that timetable properties affect punctuality, and timetables can be changed relatively quickly and easily, compared to infrastructure and rolling stock. In this paper we study some strategies for assigning margins in
Previous research has shown that timetables are important for punctuality, and research is ongoing on how to improve timetables through more simulation, optimization techniques, better scheduling of track works, and data analysis. Relatively little attention has been given to the actual planners. In this study, we have conducted interviews with timetable planners in Southern Sweden, and analyzed t
Acute coronary artery occlusion should be treated with urgent revascularization. ECG is important in the triage of patients with acute coronary syndrome. ST deviation depends on the relation between the location of the transmural ischemia and the positive poles of the 12 ECG leads. In acute occlusion of the left circumflex artery, ST elevation in two contiguous leads is not always present. InsteadAcute coronary artery occlusion should be treated with urgent revascularization. ECG is important in the triage of patients with acute coronary syndrome. ST deviation depends on the relation between the location of the transmural ischemia and the positive poles of the 12 ECG leads. In acute occlusion of the left circumflex artery, ST elevation in two contiguous leads is not always present. Instead
