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Cross-culture contamination of cell lines propagated in continuous culture is a frequent event and particularly difficult to resolve in cells expressing similar phenotypes. We demonstrate that DNA-DNA hybridization to blotted endonuclease-digested cell DNA effectively detects cross-culture contamination to monitor inter-species as well as intra-species cross contamination. An insulin-producing cel
The effect of secretin on glucagon and insulin release and its interaction with glucose has been studied in cultured mouse pancreatic islets by column perifusion. Glucose alone showed the well-known stimulation of insulin release and inhibition of glucagon release. Addition of 10 mM secretin increased glucagon secretion at 3 mM d-glucose by 300% while no change in insulin release could be seen at
Secretin and glucagon potentiate glucose-induced insulin release. We have compared the effects of secretin and glucagon with that of four hybrid molecules of the two hormones on insulin release and formation of cyclic AMP (cAMP) in isolated mouse pancreatic islets. All six peptides potentiated the release of insulin at 10mM D-glucose, and their effects were indistinguishable with respect to the dy
Neonatal insulin-dependent diabetes mellitus (IDDM) occurs rarely. A sibship of two HLA-Dw314-positive boys who developed IDDM within the 1st wk of life is described. Although the HLA-D region genotype would be consistent with IDDM associated with islet autoimmunity, islet cell antibodies were negative, but both boys exhibited the presence of a novel autoantibody that reacted specifically with a c
The acute effects of recombinant human interleukin-1 beta (rIL-1) on basal and glucose-stimulated insulin release were investigated in the isolated perfused pancreas. At a concentration of 20 μg/l rIL-1 had no effect on basal insulin release, but increased the total amount of insulin released during first and second phase insulin release in response to 20 mmol/l D-glucose in the rat pancreas (P <
Sera from 40 Swedish children diagnosed as having Type 1 (insulin-dependent) diabetes mellitus during a one year period along with 40 age and geographically matched control subjects were tested for antibodies to a Mr-64000 islet protein by immunoprecipitation of 35S-methionine-labelled rat islet amphiphilic proteins. Of the 40 diabetic patients, 29 (73%) were found to be positive whereas all 40 co
The β-cell function in the perfused pancreas, the total pancreatic insulin content, and the weights of pancreas, kidney, spleen, and total body fat were compared at the age of 30 and 45 days in diabetes-prone and body weight-matched diabetes-resistant male BB rats. These inbred rats had an incidence rate of diabetes at 90 days of age of 80 and 0%, respectively. At day 30, the pancreatic insulin co
The pattern of fall in B-cell function measured as plasma and 24 h urinary C-peptide excretion, as well as levels of islet cell antibodies, insulin antibodies and metabolic parameters, were followed for two years in 39 children aged 1-17 years prospectively from clinical onset of Type 1 (insulin-dependent) diabetes. At onset 32/36 patients had measurable plasma C-peptide (median 0.13 nmol/l). Maxi
Diabetes was induced in BALB/c mice by four injections of a subdiabetogenic dose (40 mg/kg) of streptozotocin in combination with CFA. The treatment increased the plasma glucose from 5.8 ± 0.1 to 22.1 ± 1.3 mmol/liter (n = 9). The diabetic animals had circulating islet cell surface antibodies (75%), and a monoclonal islet cell surface IgM antibody, K56aF3, generated from one of the diabetic BALB/c
Rwanda har sedan 2006 aktivt arbetat för att modernisera jordbrukssektorn med syftet att ersätta småskaliga självförsörjande jordbruk med moderna, marknadsorienterade. I denna process har småskaliga jordbrukare tvingats att överge sina traditionella grödor och jordbruksmetoder för att anpassa sig till odling i monokultur utvalt av staten på sammanslagna jordlotter.Avhandlingen, baserad på 13 månadSince 2006, Rwanda has been implementing policies to modernize the agricultural sector, with the aim of moving from small-scale subsistence farming to modern, market-oriented farming. Under these policies, small-scale farmers have been compelled to abandon their traditional farming practices and adapt to monocropping of state-approved crops on consolidated land.This dissertation, based on 13 month
Background: Blood eosinophil count (BEC) and fractional exhaled nitric oxide (FeNO) concentration are established biomarkers in asthma, associated particularly with the risk of exacerbations. We evaluated the relationship of BEC and FeNO as complementary and independent biomarkers of severe asthma exacerbations. Methods: This observational study included data from the Optimum Patient Care Research
This book offers a unique analysis of the use of the automated facial recognition algorithms that are increasingly intervening in our society from a critical visual culture studies perspective. The discussion focuses on the visuality of automated facial recognition and its designed algorithms as a case study in machinic vision and its concurrent modes of perception. It focuses on a general problem
Bacteriophages, or phages, are viruses that infect bacteria, at the end of their life cycle produce a set of enzymes called endolysins to lyse host cells from within, facilitating the release of the viral progeny. Due to their lytic activity, endolysins have gained great interest as potential antibacterials targeting both Gram–positive and –negative bacteria, especially in the actual context of in
Den statliga förvaltningen i dess olika skepnader har sedan länge mycket stor betydelse för samhället och enskilda i de nordiska länderna. En central fråga i offentlig rätt och statsvetenskap är hur förvaltningens ställning i samhället ska förstås. I ett offentligrättsligt perspektiv handlar det då bland annat om förvaltningens konstitutionella eller statsrättsliga roll. Hur fastställs de rättslig
