Double-layer vs single-layer cryogels as a dye affinity chromatography column, comparison study
Dye affinity chromatography is one of the chromatography techniques which uses dye as a ligand for protein purification. In this study, single-layer poly(acrylamide-allyl glycidyl ether) cryogel was prepared, which contains free epoxy groups at sub-zero temperature. The double-layer was prepared inside a primary single-layer cryogel to form a secondary network inside the gel. Cibacron Blue F3GA wa
