Endoglucanases (EGI) (endo-1,4-β-d-glucan-4-glucanohydrolase, EC 3.2.1.4, Cel7B) of Trichoderma reesei are industrially important enzymes. Thus, there is a great need for development of a primary recovery method suitable for large-scale utilization. In this study we present a concept applicable for large-scale purification of an EGI fusion protein by one-step extraction in a poly(ethylene glycol)

Peptidoglycan from Staphylococcus aureus mobilized CD66b in human neutrophils but did not upregulate surface activation markers in eosinophils. In addition, Toll-like receptor 2, implicated in the recognition of peptidoglycan, was detected on the surface of resting neutrophils but not on eosinophils. These findings suggest roles for neutrophils but not eosinophils in innate recognition of peptidog

Secondarily phosphatised olenid trilobites from organic-rich limestones (orsten) in the Furongian (Upper Cambrian) of Vastergotland, south-central Sweden, are described and illustrated. All trilobites originate from the Peltura scarabaeoides Zone and were collected on the western slope of Kinnekulle. Only the dorsal exoskeletons have become secondarily phosphatised, and the ventral appendages are

Popular Abstract in Swedish Studiet av entreprenøren er ikke et studium om en «helt på sin hvite hest», som er en ikke uvanlig kritikk av vellykkete næringsdrivende, men snarere en analyse av helt vanlige menneskers tanker, drømmer og liv. Entreprenørene er vanskelige å finne for de er sjeldent forekommende i befolkningen. De er viktige fordi de utfører innovasjoner, som innebærer at man blir i stThis dissertation, which is an analysis of the entrepreneur at the micro level, supplements and underpins the understanding and use of the entrepreneur as an agent of social change in the analysis of economic change at the macro level. The aim of the thesis is firstly to seek out and identify businessmen of our time who claim to be innovative, and secondly to un-dertake a qualitative study and ana

Media coverage is often held responsible for the erosion of public support for European integration. At the same time, a well-functioning political journalism is also held up as an important requirement for EU democracy. Set against this background of confl icting perceptions of the media, this report analyzes political journalism’s critical performance in the realm of EU constitutionmaking. The r

Thiazolidinediones are antidiabetic agents that decrease insulin resistance. Emerging evidence indicates that they present beneficial effects for the vasculature beyond glycemic control. The aim of this open-label observational study was to determine the effect of the thiazolidinedione rosiglitazone on novel cardiovascular risk factors, namely, lipoprotein(a) [Lp(a)], C-reactive protein (CRP), hom

Study objectives: This study was conducted to gain insight into patients' comprehension, recognition, and experience of exacerbations of COPD, and to explore the patient burden associated with these events. Design: A qualitative, multinational, cross-sectional, interview-based study. Setting: Patients' homes. Patients: Patients (n = 125) with predominantly moderate-to-very severe COPD (age >= 50 y

We compare the results of electrically detected magnetic resonance in a 2D electron gas in Si/SiGe quantum wells with transport and magnetic resonance measurements on ferromagnetic Ga1-xMnxAs. The results lead us to the conclusion that observation of electrically detected magnetic resonance is possible only in the case of a slow spin relaxation, where the microwave resonant absorption leads to a n

Human papillomaviruses (HPVs) of the genera Betapapillomavirus and Gammapapillomavirus are common on human skin. Sequencing of subgenomic amplicons of cutaneous HPVs has revealed a large number of novel putative HPV types within these genera. Phylogenetic analysis based on these amplicons revealed 133 putative HPV types with

Objective: To evaluate aspects of internal scale validity and reliability of the Swedish version of the Impact on Participation and Autonomy questionnaire (IPA-S) for use in people with spinal cord injury. Subjects: A total of 161 persons with spinal cord injury. Method. IPA-S psychometric properties were evaluated using Rasch rating scale analysis. Results: The results show that the IPA-S has 27

Chronic exposure of pancreatic islets to elevated plasma lipids ( lipotoxicity) can lead to beta-cell dysfunction, with overtime becoming irreversible. We examined, by confocal microscopy and biochemistry, whether the expression of islet inducible nitric oxide synthase ( iNOS) and the concomitant inhibition of glucose-stimulated insulin release seen after lipid infusion in rats was modulated by th

Popular Abstract in SwedishMånga skador och olägenheter av fukt kan uppkomma under en byggnads livslängd. De kan t.ex. orsaka hälsoproblem eller dålig ekonomi. För att minska fuktproblem kan en fuktdimensionering göras. I en fuktdimensionering behövs olika fuktberäkningar för att se hur fukttillståndet förväntas bli i eller på en yta av en konstruktion. Beräknade fukttillstånd används för att kontDetermination of the design conditions for moisture resistance requires in a Moisture Design number of different moisture calculations to be made in order to determine the moisture status within, or on the surface of, a structure. The objective of this work has been to ascertain how to allow for variations in climate data in order to be able to determine the most unfavourable moisture status likel

Rapport från en internationell konferens i Montreal 11-13 oktober 1998 "The First International Conference on New Information Technologies for Decision Making in Civil Engineering". Författaren (som är bibliotekarie) var den enda representanten från Sverige bland ca 280 forskare från 26 länder, och berättar om sitt eget bidrag: "CER: A Handy Tool for the Civil Engineer", men tar även hjälp av 5 ol

Background: Flat and depressed colorectal tumours are common in Japan but are very rare or non-existent in Western countries. Aims: To study the occurrence of flat colorectal tumours in a southern Swedish population. Methods: In this prospective study, 371 consecutive European patients were examined by high resolution video colonoscopy combined with chromoendoscopy. The nature of the lesions was

Secretagogin is a hexa EF-hand protein, which has been identified as a novel potential tumour marker. In the present study, we show that secretagogin binds four Ca2+ ions (log K-1 = 7.1 +/- 0.4, log K-2 = 4.7 +/- 0.6, log K-3 = 3.6 +/- 0.7 and log K-4 = 4.6 +/- 0.6 in physiological salt buffers) with a [Ca2+](0.5) of approx. 25 mu M. The tertiary structure of secretagogin changes significantly upo