PCR-based detection of microorganisms in complex biological samples
The detection of microorganisms by PCR can be divided into four steps: (1) sample collection, (2) sample preparation, (3) DNA amplification and (4) detection of PCR products. The major problem in developing PCR-based detection methods is the sample preparation step. This step is necessary to overcome problems caused by PCR inhibitors and it determines, to a large extent, the sensitivity of the PCR