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PCR-based detection of microorganisms in complex biological samples

The detection of microorganisms by PCR can be divided into four steps: (1) sample collection, (2) sample preparation, (3) DNA amplification and (4) detection of PCR products. The major problem in developing PCR-based detection methods is the sample preparation step. This step is necessary to overcome problems caused by PCR inhibitors and it determines, to a large extent, the sensitivity of the PCR

B2B-Marketplace Value Creation, Conceptual Predictions and Empirical Findings

Information technology is a vital driver in today’s business environment. Recent developments of IT have facilitated for the relatively easy creation of markets on the Internet, so called B2B-marketplaces. However, judging from the survival rate of B2B-marketplaces (5 to 10 per cent), it seems like B2B-marketplaces are doing something wrong. This paper scrutinises several theoretical frameworks re

Lighting Recommendations in Operating Theatres

“The eyes lead the body.” A good visual environment is important for performance and wellbeing. Unsatisfactory visual conditions often induce a strained posture, more or less unconsciously, in an effort to improve vision. The requirements for the visual environment in an operating theatre are high. Surgeons or assistants who are having visual problems can induce treatment hazards. The purpose of t

Solar control of meteor radar rates

Meteor radar observations carried out in August-September 1953-84 at theOnsala Space Observatory in Sweden show long-term, as well as short termvariations in meteor radar rates. These rate variations are associatedwith position in the solar cycle, and with variations in the solar windsector structure. An inverse correlation is found between meteor radarrates and the solar cycle. The magnitude of t

Therapeutic aspects of oral and pharyngeal swallowing dysfunction. Videoradiographic and videomanometric analyses of adult healthy volunteers and dysphagic patients.

Popular Abstract in Swedish Dysfagi är ett symptom på onormal sväljningsförmåga. Detta symptom kan förekomma i samband med många olika sjukdomstillstånd men är vanligast i samband med olika neurologiska sjukdomar t ex stroke. Symptomen kan variera från en lätt känsla av upphakning och svårigheter att svälja specifik föda till livshotande kvävningsanfall. Logopeder har de senaste decennierna tagit In this study simultaneous videoradiography and intraluminal manometry (videomanometry) have been used in combination in three studies to analyze how different therapeutic strategies affect the physiology of swallowing in healthy volunteers and in patients with pharyngeal dysfunction. Analyses of healthy volunteers showed few significant measurable effects on the pharyngeal swallow. Some decreased

Skall äldre särbehandlas?

Allt fler får uppleva sin 100-årsdag. I hela Sverige finns det drygt 2 000 personer som är 100 år eller äldre. På fyrtio år har antalet personer över 65 år ökat med 57 %, från 1,17 miljoner 1972 till 1,83 miljoner 2012 – detta samtidigt som den totala folkmängden bara ökat med 17 %. Vad innebär det att åldras i Sverige? Sverige är ett av de få länder som har en äldreomsorg som omfattar alla oavset

Kyrkorum och bild i Brunnby kyrka

Ever since Brunnby Church was first erected it has been altered and it has crossed boundaries in terms of both religious and national affiliation - from a Catholic to a Lutheran place of worship and from being a Danish to being a Swedish church. The boundaries around and within the church itself have changed, and have become displaced as new forms of worship have been introduced. Boundaries and in

Miljöpraktiker i vardagen: Livsstil, information och förändring i Simrishamn

Miljö och miljöfrågor har under den senaste tiden lyfts fram som ytterst viktiga för framtiden, inte minst växthuseffekten och dess konsekvenser. Att vara och agera miljömedvetet kan sägas ha gått från att ha varit ett val för ett engagerat fåtal till att ha blivit en etablerad livsstil för många. Miljöfrågor är också av stor betydelse för Simrishamns kommun. Under några sommarmånader mer än fördu

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Abstract in Arabic Gå till historien är en bok på arabiska med sex tematiska vandringar genom Malmö.

Control of fed-batch bioreactors. Part II

This chapter addresses the control of glucose feeding in fed-batchreactors. The feeding strategy is based on a pulse technique, whichdoes not require a priori knowledge of the process. A feedbackalgorithm optimizes the feed rate with respect to the constraints ofaerobic conditions and overfeeding. Stability and performance analysisare performed on a simplified model of the reactor, using standard

Telomeric DNA in Chironomus, a naturally telomerase-free system

Popular Abstract in Swedish Kromosomer, långa linjära DNA molekyler som finns i eukaryota organismers cellkärna i komplex med proteiner, är bärare av genetisk information. Kromosomer är samlingar av gener men de innehåller också regioner utan gener som är oumbärliga för kromosomstabiliteten och för att gener ska fördelas jämt vid varje celldelning. De strukturer som finns i slutet av linjära kromoTelomeres are specialized complexes of DNA and associated proteins that protect the ends of eukaryotic chromosomes. Although most organisms have short repeated DNA sequences at their telomeres, which are specified and maintained by the enzyme telomerase, there are a few exceptions. Dipteran insects, including the genus Chironomus (non-biting midges), lack telomerase and have atypical terminal sequ

A Quantum Chemical Study of Hydrogen Abstraction from Manganese-Coordinated Water by a Tyrosyl Radical: A Model for Water Oxidation in Photosystem II

Recently, water oxidation in photosystem II was proposed to involve direct abstraction of hydrogen atoms from water molecules terminally ligated to manganese ions in the oxygen-evolving complex by the oxidized tyrosine radical, TyrZ. This model is tested here by performing quantum chemical calculations. An empirically parametrized hybrid density functional method is used, and both monomeric and di