he present study focused on the functional role of the mutation Ile66Thr located in the N-terminal epidermal growth factor-like domain of coagulation factor IX (FIX). This mutation causes mild hemophilia B with approximately 25% FIX coagulant activity and FIX antigen levels of around 90% of normal. In the 3-dimensional structure of porcine FIXa and in the subsequent 3-dimensional model of human FI

BACKGROUND: Electrophoresis of serum samples allows detection of monoclonal gammopathies indicative of multiple myeloma, Waldenstrom macroglobulinemia, monoclonal gammopathy of undetermined significance, and amyloidosis. Present methods of high-resolution agarose gel electrophoresis (HRAGE) and immunofixation electrophoresis (IFE) are manual and labor-intensive. Capillary zone electrophoresis (CZE

BACKGROUND: It is important to clarify the biological function of the female sex hormones estrogen and progesterone in periodontal ligament cells, as these hormones may affect periodontal health. We have previously shown that human periodontal ligament cells express estrogen receptor beta (ERbeta) but not ERalpha, whereas human breast cancer cells (MCF7) express both ERalpha and ERbeta. Data on pr

The northern pike (Esox lucius) is an important and selective piscivore that chooses smaller prey than predicted from energy / time budgets. In a laboratory experiment, we investigated pike predatory behavior to explain this selectivity. Northern pike feeding on different prey sizes in aquaria were observed when foraging alone, when in the presence of chemical cues from similar-sized or larger con

The degradation of extracellular matrix during cancer invasion results from the action of several protease and protease inhibitor systems. alpha(1)-Antitrypsin (AAT) is a serine proteinase inhibitor produced by various tumour cells, and its plasma concentration rises during inflammation, infection and malignant diseases. AAT is found in a native, inhibitory active form, but also in other, non-inhi

Various techniques are available for the conversion of lignocellulosics to fuel ethanol. During the last decade processes based on enzymatic hydrolysis of cellulose have been investigated more extensively, showing good yield on both hardwood and softwood. The cellulase production of a filamentous fungi, Trichoderma reesei Rut C 30, was examined on carbon sources obtained after steam pretreatment o

BACKGROUND: The purpose of this study was to validate the use of whole-blood samples in the determination of circulating forms of prostate-specific antigen (PSA). METHODS: Blood samples of hospitalized prostate cancer and benign prostatic hyperplasia patients were collected and processed to generate whole-blood and serum samples. Three different rapid two-site immunoassays were developed to measur

We show that the difference in the infrared vibrational spectrum of C-H stretch modes arising from the methyl group of two surface intermediates, methylthiolate and methoxy, originates from the difference in the methyl deformation modes frequencies of the two intermediates. Importantly, while the vibrational fingerprint of the two methyl groups are not transferable between the intermediates, the F

The Euro50 is a proposed 50m extremely large telescope for optical and infrared wavelengths. To study and predict the performance of the complete telescope system, an integrated model combining the structural model of the telescope, optics models, the control systems and the adaptive optics has been established. Wind and atmospheric disturbances are also included in the model. The model is written

BACKGROUND/AIMS: This study aimed to determine whether deficiency of the major serine protease inhibitors (alpha1-antitrypsin (AAT) or alpha1-antichymotrypsin (ACT)) is associated with increased risk for chronic hepatitis B or C virus (HBV or HCV) infection. METHODS: We studied 709 adults with chronic liver disease who had undergone liver biopsy during the 14-year period 1978-92. Anti-HCV testing

One interpretation of the model considered in this paper is a single-echelon inventory system consisting of a number of local warehouses, which normally replenish from an outside supplier. In case of a stockout at a warehouse an emergency lateral transshipment from another warehouse may be possible. However, such transshipments are only allowed in one direction, i.e., the flow structure is unidire

Serum concentrations of CB-153 and pp'-DDE were assessed for 354 men and women from the Swedish Fishermen's Families Cohort, and were found to correlate very well (Pearson's r = 0.72). In this particular cohort the main source of exposure to persistent organochlorine compounds are consumption of contaminated fatty fish. High correlations between total PCB/CB-153 and p,p'-DDE have also been found i

The substrates required for glycolysis change markedly at successive stages of spermatogenesis suggesting a considerable plasticity in the expression of glycolytic enzymes. Lactate dehydrogenase (LDH) isoenzymes, LDH-A and LDH-B, are expressed in premeiotic, meiotic cells, and early spermatids, both in avian and mammalian spermatogenesis. Highly polyadenylated forms, particularly of LDH-A, were de