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Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen

Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis f

Magnetization orientation dependence of the quasiparticle spectrum and hysteresis in ferromagnetic metal nanoparticles

We use a microscopic Slater-Koster tight-binding model with short-range exchange and atomic spin-orbit interactions that realistically captures generic features of ferromagnetic metal nanoparticles to address the mesoscopic physics of magnetocrystalline anisotropy and hysteresis in nanoparticle-quasiparticle excitation spectra. Our analysis is based on qualitative arguments supported by self-consi

NADH electrocatalytic oxidation at glassy carbon paste electrodes modified with meldola blue adsorbed on acidic alpha-zirconium phosphate

The electrochemical behavior and the electrocatalytic activity for the NADH oxidation at glassy carbon paste electrodes (GCPEs) modified with Meldola Blue (MB) adsorbed onto acidic cc-zirconium phosphate (ZP*) have been investigated. Cyclic voltammetry and rotating disc electrode measurements, performed in Tris buffer solution (pH 7) in absence and in presence of different NADH concentrations, sho

Microbial Desulfurization of Coal and Oxidation of Pure Pyrite by T. ferrooxidans and Acidianus brierleyi

Thiobacillus ferrooxidans and Acidianus brierleyi were capable of oxidizing pure pyrite as well as oxidizing sulfur in coal. First order reactions were assumed in the kinetic analysis performed. For oxidation of pure pyrite the rate constant was higher for A. brierleyi than for T. ferrooxidans. For sulfur removal from coal the values of the rate constants were comparable far the two microorganisms

Neutral dissociation of super excited states in nitric oxide

Near-infrared dispersed fluorescence measurements of 13 different atomic multiplets of neutral atomic fragments from photon induced neutral dissociation processes in NO are reported. For excitation of the molecules narrow band synchrotron photons of energy 17.2-25.8 eV were used. Neither Rydberg series nor other molecular states in NO known so far can account for the collected data. From ab initio

Attenuation of earplugs - Objective predictions compared to subjective REAT measurements

By using an experimental setup yielding the acoustical Two-Port properties of earplugs. the attenuation of three fundamentally different types of earplugs is predicted. The setup used is based on molds of the ear canals of a real person, and the Two-Port of each earplug is numerically coupled to an acoustical model of the ear. The predicted attenuation is compared to the subjective attenuation (RE

Aqueous two-phase systems containing self-associating block copolymers - Partitioning of hydrophilic and hydrophobic biomolecules

A series of proteins and one membrane-bound peptide have been partitioned in aqueous two-phase systems consisting of micelle-forming block copolymers from the family of Pluronic block copolymers as one polymer component and dextran T500 as the other component. The Pluronic molecule is a triblock copolymer of the type PEO-PPO-PEO, where PEO and PPO are poly(ethylene oxide) and poly(propylene oxide)

Accidental mydriasis from exposure to Angel's trumpet (Datura suaveolens).

PURPOSE: To report clinical findings after accidental instillation into the eye of sap from Angel's trumpet (Datura suaveolens). METHODS: We report findings on seven patients who developed sudden onset of unilateral mydriasis. At least three of them also had ipsilateral cycloplegia and one developed transient tachycardia. RESULTS: The symptoms evolved after ocular exposure to sap from Angel's trum

Computational analysis of an O2separating membrane for a CO2-emission-free power process

The increased demand for clean power in recent years has led to the development of various processes that include different types of CO2 capture. Several options are possible: pre-combustion concepts (fuel de-carbonization and subsequent combustion of H2), post-combustion concepts (tail-end CO2 capture solutions, such as amine scrubbing), and integrated concepts in which combustion is carried out

Immunohistochemical analysis of glioma-infiltrating leucocytes after peripheral therapeutic immunization with interferon-gamma-transfected glioma cells

We have shown previously that rejection of preinduced rat brain tumours is possible following therapeutic immunizations with interferon-gamma (IFN gamma)-transfected glioma cells (N32-IFN gamma). In the present study we have used the same model to evaluate whether quantitative differences in tumour-infiltrating lymphocytes can be detected between animals receiving therapeutic immunizations with ei