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The gene encoding Amy 34, a maltohexaose-forming α-amylase from Bacillus halodurans LBK 34 isolated from Lake Bogoria, Kenya, was cloned and sequenced. The mature peptide consists of 958 amino acids with a theoretical molecular weight of 107.2 kDa and pI 4.41, respectively. The gene was expressed in Escherichia coli and the recombinant enzyme purified to homogeneity by a combination of metal chela
The pied flycatcher is one of the most phenotypically variable bird species in Europe. The geographic variation in phenotypes has often been attributed to spatial variation in selection regimes that is associated with the presence or absence of the congeneric collared flycatcher. Spatial variation in phenotypes could however also be generated by spatially restricted gene flow and genetic drift. We
Excited states in the N = Z+1 nucleus Y-79 Were identified using the reaction Si-28(Fe-54, p2n)Y-79 at a 200 MeV beam energy and an experimental set up consisting of an array of Ge detectors and the Recoil Mass Spectrometer at Oak Ridge National Laboratory. With the help of additional gamma-gamma coincidence data obtained with Gammasphere, these gamma-rays were found to form a strongly-coupled rot
A calorimetric method for the study of solid-vapor interactions is described. In one calorimetric chamber a vapor evaporates; in a second calorimetric chamber the vapor is absorbed by a sample. The two chambers are connected by a tube and form part of a double twin microcalorimeter. As vapor is sorbed by the sample the vapor pressure in the sorption chamber will increase from a low value to near s
Photoactivation of photosystem II has been studied in the FUD 39 mutant of Chlamydomonas reinhardtii that lacks the 23 kDa extrinsic subunit of photosystem II. We have taken advantage of the slow photoactivation rate of FUD 39, earlier demonstrated in Rova, E. M., et al. [(1996) J. Biol. Chem. 271, 28918-28924], to study events in photosystem II during intermediate stages of the process. By measur
Background: Matrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix is a key point in tumor development and expansion. MMP-10 is one of the most important and well-characterized members of the MMP family. In the present study, we examined MMP-10 mRNA and protein levels in non-small cell lung cancer (NSCLC). Patients and Methods: Three endogenous reference genes including GA
Background and Purpose: Leptin, an important hormone for body weight regulation, may be involved in the pathogenesis of cardiovascular manifestations of obesity. We tested whether leptin may be an independent risk marker for stroke in a case-referent study. Methods: Definitive acute stroke events, defined by MONICA criteria, were identified from October 1, 1995 to April 30, 1999. Referents without
Many shorebirds travel over large sections of the globe during the course of their annual cycle and use habitats in many different biomes and climate zones. Increasing knowledge of the factors driving variations in shorebird numbers, phenotype and behaviour may allow shorebirds to serve as 'integrative sentinels' of global environmental change. On the basis of numbers, timing of migration, plumage
Two new thermostable alpha-L-rhamnosidases with novel substrate hydrolysis pattern were cloned and expressed from a new thermophilic bacterium. Fragments of the two alpha-L-rhamnosidase genes, rhmA and rhmB were identified in a partially sequenced genome of the bacterium. Whole genes were recovered by amplifying flanking sequences with single specific primers and nonspecific walking primers. The r
Laminins are alphabetagamma heterotrimeric extracellular proteins that regulate cellular functions by adhesion to integrin and nonintegrin receptors. Laminins containing alpha4 and alpha5 chains are expressed in bone marrow, but their interactions with hematopoietic progenitors are unknown. We studied human bone marrow cell adhesion to laminin-10/11 (alpha5beta1gamma1/alpha5beta2gamma1), laminin-8
Non-word repetition has, not without controversy, been considered a reliable index of phonological memory in children with language impairment (LI). Recent studies do, however, emphasize the strong link between non-word repetition skills and phonological output in pre-school children with LI. The purpose of the present study was to explore the relationship between non-word repetition and different
In this article, we present the second case of a tongue carcinoma with distinct morphological similarities to a primary sclerosing sweat duct carcinoma of the skin. The tumor infiltrated deeply into the striated muscle and exhibited extensive peri- and intraneural growth. Neoplastic cells had mildly pleomorphic nuclei and displayed in addition to small cysts, distinct duct formation with a bilayer
Carbon monoxide (CO), carbon dioxide (CO2), and radiocarbon (14CO2) measurements have been made in Heidelberg from 2001 to 2004 in order to determine the regional fossil fuel CO2 component and to investigate the application of CO as a quantitative tracer for fossil fuel CO2 (CO2(foss)). The observations were compared with model estimates simulated with the regional transport model REMO at 0.5° × 0
Objective. This study was undertaken to investigate the previously reported association of the STAT4 polymorphism rs7574865 with rheumatoid arthritis (RA) in 3 different European populations from Spain, Sweden, and The Netherlands, comprising a total of 2,072 patients and 2,474 controls. Methods. Three different cohorts were included in the study: 923 RA patients and 1,296 healthy controls from Sp
In this paper we prove the local existence of complex-valued harmonic morphisms from any compact semisimple Lie group and their non-compact duals. These include all Riemannian symmetric spaces of types II and IV. We produce a variety of concrete harmonic morphisms from the classical compact simple Lie groups SO(n), SU(n), Sp(n) and globally defined solutions on their non-compact duals SO(n, C)/SO(
Inhibition of dipeptidyl peptidase-4 (DPP-4) is currently explored as a novel therapy of type 2 diabetes. The strategy has been shown to improve glycemia in most, but not all, rodent forms of glucose intolerance. In this study, we explored the effects of DPP-4 inhibition in mice with [beta-cell overexpression of human islet amyloid polypeptide (IAPP). We therefore administered the orally active an
Ectomycorrhizal mycelial systems were grown in unsterile soil using specially designed growth chambers, and the respiratory activity of the mycelium was measured before and after its excision from the host roots. Approximately 30% of the total respiration in the intact system was attributable to the mycorrhizal mycelium in the soil. Severance of mycelial connections at the roots lead to a greater
