Immobilisation procedure and reaction conditions for optimal performance of Candida antarctica lipase B in transesterification and hydrolysis
The reaction kinetics of Candida antarctica lipase B (CalB) in the commercially available preparation Novozym (R) 435 (N435) were compared to those of preparations of CalB immobilised on Accurel (R) MP1000 (porous polypropylene). Two polypropylene preparations were made using enzyme loadings of 0.2% and 2% (w/w). All three preparations were used in hydrolysis as well as transesterification of two