We use cryo electron microscopy (cryo-EM) and image reconstruction to examine the virion structure at subnanometer resolution. We also analyze structure and density of viral DNA packaged in a capsid.  Figure: Cutaway views from cryo-EM reconstructions of (A) HSV-1 C-capsid and (B) phage lambda procapsid and mature DNA-filled lambda capsid. HSV-1 and lambda phage capsids are shown to scale. Owing t

https://www.virus-biophysics.lu.se/methods-and-techniques/cryo-electron-microscopy - 2025-04-25

The isothermal titration calorimetry (ITC) provides the most direct method to measure the internal energy of the confined viral genome. In order to experimentally dissect the thermodynamic determinants of viral DNA packaging and ejection energetics, we were first to use ITC to directly measure the heat released by DNA ejection from a virus capsid. This approach allows one to address experimentally

https://www.virus-biophysics.lu.se/methods-and-techniques/microcalorimetry - 2025-04-25

Super-Resolution Structured Illumination Microscopy (SR-SIM) provides resolutions down to 120 nm, allowing visualization of individual herpesvirus capsids (HSV-1) attached to the nucleus (HSV-1 C-capsid diameter is 125 nm).  Figure: Imaging of reconstituted capsid-nuclei system confirms specific capsid binding to the NPCs at the nuclear membrane. Representative super-resolution SIM image showing G

https://www.virus-biophysics.lu.se/methods-and-techniques/super-resolution-and-single-particle-fluorescence-microscopy - 2025-04-25

Despite the structure of packaged viral DNA being central for ejection and consequentially infectivity of the virus, the detailed understanding of the packing structure of DNA inside the viral capsid. SAXS and SANS allow investigation of the structure of DNA filled capsids in solution in a wide range of environmental conditions. SAXS and SANS are powerful techniques that provides detailed structur

https://www.virus-biophysics.lu.se/methods-and-techniques/small-angle-x-ray-scattering-saxs-and-small-angle-neutron-scattering-sans-viral-genomes - 2025-04-25

For many double stranded (ds) DNA viruses the viral genome is actively packaged into the virus capsid by a powerful molecular motor. This results in an energetically unfavorable state of the packaged genome, creating tens of atmospheres internal pressure inside the virion. Using a new calorimetric assay to determine the activation energy barriers associated with genome loss in response to elevated

https://www.virus-biophysics.lu.se/covers-highlights - 2025-04-25

Följ det Vetenskapsrådsfinansierade forskningsprojektet ”Döden på Vipeholm – en undersökning om vården av ’osnygga obildbara sinnesslöa’ och möjlig medveten dödssvält”. Om projektet Under några av 1940-talets första år dog 200 av de patienter som bodde på Vipeholms sjukhus. Vad berodde det på, att så många dog under de här åren? Kan det ha handlat om eutanasi – vilket har framförts men aldrig lett

https://www.vipeholm.lu.se/vipeholm - 2025-04-25

Artikel av Kristina Engwall i Nordic Journal of Educational History”… äro absolut obildbara”: Barnen på Vipeholm 1935–1963DOI: https://doi.org/10.36368/njedh.v11i1.456Abstract: Hospital in Sweden was the only institution for the ’uneducated, difficult to care for feebleminded’. Although it was designed for adults, a fifth of those admitted were under the age of 15. The article focuses on the child

https://www.vipeholm.lu.se/om-projektet/publicerat - 2025-04-25

Elin Bommenel skriver om sjukhuschefen och överläkaren Hugo Fröderberg Under sitt långa yrkesliv fick Hugo Fröderberg (1897–1986) följa utvecklingen av vad som då kallades ”sinnesslövården”. För eftervärlden kom han att bli känd som sjukhuschef och överläkare på Vipeholms sjukhus – Europas största i sitt slag med plats för nästan 1000 ”svårskötta, obildbara sinnesslöa”. Hugo Fröderberg föddes i Bä

https://www.vipeholm.lu.se/vem-var-hugo-froderberg-och-vad-var-vipeholms-sjukhus - 2025-04-25

Ett tvärvetenskapligt forskningsprojekt om Vipeholm Under krigsåren 1941–1943 dog ett ovanligt stort antal intagna på Vipeholm. Varför vet vi inte med säkerhet ännu. Genom tvärvetenskaplig arkivforskning arbetar fyra forskare med att ta reda på vad som egentligen hände. Hur tar ett välfärdssamhälle hand om sina marginaliserade, mest sårbara invånare? Och hur har synen förändrats på de människor so

https://www.vipeholm.lu.se/om-projektet - 2025-04-25

De deltar i forskningsprojektet ”Döden på Vipeholm” En historiker, en funktionshinderforskare, en idéhistoriker och en medicine doktor specialiserad i psykiatri. Läs mer om deltagarna i det tvärvetenskapliga forskningsprojektet. Foto: Octar Guloglu Elin Bommenel är lektor i Service management och tjänstevetenskap vid Lunds universitet. Där undervisar hon om health management samt om vetenskapsteor

https://www.vipeholm.lu.se/om-projektet/medverkande - 2025-04-25

Research at the Division The Division of Particle and Nuclear Physics spans broad across subatomic physics and acts largely within international research environments. We conduct experimental and theoretical research with collider experiments. We are engaged in dark-matter searches, fundamental physics with neutrons, nuclear structure and nuclear reactions at the limits of nuclear stability, as we

https://www.particle-nuclear.lu.se/welcome - 2025-04-25

The Theoretical Particle Physics group is mainly active in particle physics phenomenology. Work which relates theory to quantitative predictions for collider experiments. The group is subdivided into smaller, more informal, research groups centered around different research areas, which again are clustered into different categories, centered around the individual researchers' projects. They includ

https://www.particle-nuclear.lu.se/theoretical-particle-physics - 2025-04-25

The theory group in Lund has been a pioneer in the field of event generators for high energy physics, with particular emphasis on Quantum Chromodynamics (QCD), since the inception of the field in the late 1970s. Today seven seniors, Christian Bierlich, Rikkert Frederix, Gösta Gustafson (emeritus), Leif Lönnblad, Malin Sjödahl, Torbjörn Sjöstrand (emeritus) and Korinna Zapp all work on various aspe

https://www.particle-nuclear.lu.se/theoretical-particle-physics/qcd-and-eg - 2025-04-25

The PYTHIA Monte Carlo event generator is one of the most widely used pieces of event generator software in the world. Originally a Lund University project, it is now maintained by an international collaboration with members from around the world. The PYTHIA program aims to deliver a full description of all aspects of collisions between particles such as electrons, protons, photons and heavy nucle

https://www.particle-nuclear.lu.se/theoretical-research-areas/qcd-for-mc/pythia - 2025-04-25

Low-energy particle physics is an area of particle physics covering most things not done at the highest energies. It encompasses what goes under the names of flavour-, precision- and hadronic physics and has strong connections to some areas of nuclear physics. Within the Lund theory group, Johan Bijnens, together with students and post docs, works on projects within all these aspects. In the histo

https://www.particle-nuclear.lu.se/theoretical-particle-physics/low-energy - 2025-04-25

Physics beyond the Standard Model refers to the search for a phenomenologically consistent theoretical framework providing a first-principle explanation of the known properties and deficiencies of the Standard Model being a low-energy effective field theory limit of a more fundamental ultraviolet-complete theory. Currently the group consists of two seniors: Roman Pasechnik and Johan Rathsman. Desp

https://www.particle-nuclear.lu.se/theoretical-particle-physics/bsm - 2025-04-25

Bachelor's and master's degree projects at the Division of Particle and Nuclear Physics The Division welcomes both bachelor and master students to do their degree project in one of our research groups. Below you can find short descriptions of possible topics for your degree project with links to the respective research groups for more information. You can also contact teachers directly and ask abo

https://www.particle-nuclear.lu.se/education/degree-projects - 2025-04-25

General instructions for the Java applicationsAll the applications are written using the Java language. This has the advantage that they can be run on any platform but means they need to have a Java Runtime Environment (JRE) installed. This can be freely downloaded from the Oracle site that has versions for different platforms. Accept the licence agreement and download the relevant JRE.Mac OSX: Do

https://www.particle-nuclear.lu.se/lars-gislen/downloads - 2025-04-25

The division organises regular seminars, traditionally known as Science Coffee, usually alternating between experimental and theoretical research topics. Science coffee seminars can sometimes be replaced with informal seminars, usually on topics with a more narrow scope. The normal starting time is Tuesdays at 11:15. Organizers are Alice Ohlson (experiment) and Roman Pasechnik (theory).  Seminar c

https://www.particle-nuclear.lu.se/events/division-seminars - 2025-04-25

Bachelor and Master projects Computing in particle physics experiments Modern Particle Physics is also appropriately called High Energy Physics, because it relies upon accelerators delivering high-energy particle collisions in order to generate data. Gone are the days when particle physicists were climbing mountains with photographic plates, hoping to register rare particles coming from collisions

https://www.particle-nuclear.lu.se/experimental-particle-physics/escience/degree-projects-computing - 2025-04-25